A preparation method of chitosan derivative nanoparticles delivering siRNA

A chitosan derivative and nanoparticle technology, applied in the field of gene biology, to achieve the effect of promoting the release of siRNA, inhibiting proliferation and migration, and the method is simple
CN111840575BActive Publication Date: 2022-02-11HEFEI UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HEFEI UNIV OF TECH
Publication Date
2022-02-11

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Abstract

The invention relates to a preparation method of chitosan derivative nanoparticles for delivering siRNA, belonging to the technical field of gene biology. The present invention mixes positively charged chitosan hydrochloride solution with siRNA solution that can inhibit colon cancer cell proliferation and migration-related protein expression, and then adds negatively charged carboxymethyl chitosan solution, through intermolecular electrostatic interaction function and self-assemble into chitosan-derived nanoparticles (siRNA‑CDNPs) for delivery of siRNA. The chitosan derivative nanoparticles for delivering siRNA can effectively protect siRNA under the condition of pH value 1.2, and realize the controllable release of siRNA by responding to external stimuli (ultrasound) under the condition of pH value 5.5 in the environment of colon cancer cells ; siRNA‑CDNPs can effectively inhibit the expression of β‑catenin protein in colon cancer cells, and is expected to inhibit the proliferation and migration of cancer cells. The preparation method of the invention is simple to operate, environmentally friendly and low in cost.
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Description

technical field

[0001] The invention belongs to the technical field of gene biology, and in particular relates to the preparation and application of a pH-sensitive siRNA / chitosan derivative nano system. Background technique

[0002] Gene-based therapeutic approaches hold great potential to modulate the silencing of large numbers of target mRNA molecules, ultimately reducing target protein levels. However, the application of this gene silencing technology still needs to overcome challenges, including the rapid degradation of siRNA under physiological conditions and the difficulty of crossing the negatively charged plasma membrane. A key challenge in achieving siRNA therapeutic efficacy is the development and design of novel degradable vectors with safe and sufficient gene delivery efficacy. Currently, the 5-year survival rate for patients with colon cancer liver metastases treated with chemotherapy or resection is less than 50%. It is generally believed that compared with r...

Claims

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