Gene alternative splicing analysis method

An analysis method and gene technology, applied in the field of bioinformatics, can solve problems such as variable shear analysis that has not been specifically disclosed

Active Publication Date: 2020-10-30
SHENZHEN UNIV +1
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Problems solved by technology

[0009] How to perform variable splicing analysis is not specifically disclosed in the existing technology, so a gene variable splicing analysis method is needed to realize the analysis of the variable splicing of mature transcripts

Method used

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Embodiment Construction

[0044] In order to make the purpose, technical solution and advantages of the present application clearer, the present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present application, and are not intended to limit the present application. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without making creative efforts belong to the scope of protection of the present application.

[0045] Transcript is one or more mature messenger ribonucleic acid (messenger Ribonucleic Acid, mRNA) formed by the transcription of a gene that can encode protein.

[0046] At present, gene sequencing technology is often used when studying transcripts. Gene sequencing technology is a genetic testing technology that is developing rapidly and has developed to the fourth ...

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Abstract

The invention is applicable to the technical field of biological information, and provides a gene alternative splicing analysis method. The method comprises the following steps: acquiring first original sequencing data to be analyzed; performing data preprocessing operation on the first original sequencing data to obtain first clean data after data preprocessing; performing quality detection on the first cleaning data, and if the first quality result is that the quality is qualified, obtaining a reference genome and a gene annotation file of the reference genome; performing sequence alignmenton the first cleaning data and the reference genome to obtain a first sequence alignment result; and analyzing the first sequence comparison result according to the gene annotation file of the reference genome to obtain an alternative splicing analysis result of the first target sample. According to the embodiment of the invention, the analysis of the alternative splicing condition of a mature transcript and the analysis of the differential alternative splicing of the transcript under specific conditions are realized.

Description

technical field [0001] The present application belongs to the field of bioinformatics technology, and in particular relates to a gene variable shearing analysis method based on transcriptome sequencing. Background technique [0002] Transcripts are one or more mature messenger ribonucleic acids (messenger Ribonucleic Acid, mRNA) formed by the transcription of a gene that can encode proteins. [0003] At present, gene sequencing technology is often used when studying transcripts. Gene sequencing technology is a genetic testing technology that is developing rapidly and has developed to the fourth generation of sequencing technology. Considering factors such as material sources and research costs, researchers often use next-generation sequencing technology. [0004] Next-generation sequencing technology is mainly divided into three types, that is, using Illumina's Solexa platform, Roche Applied Science's 454system and Applied Biosystems' SOLiD platform for sequencing. Among ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/30
CPCG16B20/30
Inventor 林泽腾田鹏黄腾波
Owner SHENZHEN UNIV
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