A kind of red lotos subgenus water lily pigment and its extraction method and application
An extraction method, red technology, applied in the direction of application, chemical instruments and methods, botany equipment and methods, etc., to achieve the effect of easy operation, high extraction rate and simple steps
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Embodiment 1
[0056] This embodiment tests the extraction effect of different extractants
[0057] Distilled water, 60% ethanol aqueous solution, dehydrated alcohol, dehydrated methanol, 60% ethanol-acid mixed solution (containing 0.2mol / l HCl) five kinds of extractants are set respectively, after the dry pollen of Indian red petals and extractant are mixed, Ultrasonic treatment and microwave treatment are carried out to separate the solid and liquid of the treatment liquid to obtain the pigment extraction liquid; the solid-liquid ratio is fixed at 1:40 (g / mL), the ultrasonic time is 2min, the microwave temperature is 40°C, the microwave time is 5min, and the microwave power is 400W. The extraction effect is shown in Table 1 and figure 1 , figure 1 From left to right in the middle are the extraction effects of anhydrous methanol, anhydrous ethanol, distilled water, 60% ethanol aqueous solution, and 60% ethanol-acid mixed solution.
[0058] The extraction effect of different extractants in...
Embodiment 2
[0062] This embodiment tests the extraction effect of different ethanol-acid mixed solutions
[0063] Extractant preparation:
[0064] 60% ethanol-hydrochloric acid mixed solution: the volume fraction is 60% ethanol aqueous solution and 12mol / L concentrated hydrochloric acid are mixed according to the volume ratio of 59:1, and the obtained solution is recorded as 60% ethanol-hydrochloric acid, wherein the concentration of hydrochloric acid is 0.2mol / L;
[0065] 60% ethanol-citric acid mixed solution: mix 60% ethanol aqueous solution and citric acid by volume fraction, control the content of citric acid in every 100mL mixed solution to be 1.0g, and record the resulting solution as 60% ethanol-citric acid;
[0066] 60% ethanol-oxalic acid mixed solution: mix 60% ethanol aqueous solution and oxalic acid, control the content of oxalic acid in every 100mL mixed solution to 1.0g, and record the obtained solution as 60% ethanol-oxalic acid;
[0067] Set respectively 60% ethanol-hy...
Embodiment 3
[0071] Screening of reaction conditions for the determination of anthocyanins in ethanol-oxalic acid extract by pH differential method:
[0072]1. Screening for the optimum pH: Anthocyanins are stable in an acidic environment, and their absorbance value changes with the change of pH value. Two stable pH values, the absorbance value detected at the pH value with the largest difference between the two, can be calculated by the corresponding formula to obtain the most accurate determination result of anthocyanin content. Prepare 13 gradient buffer solutions with pH of 0.5, 0.8, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, take 13 10mL volumetric flasks, and draw the pigment extract (extract The agent is 60% ethanol-oxalic acid (the concentration of oxalic acid is 0.01g / mL), and the extraction conditions are consistent with Example 2) 0.2mL is placed in the bottle, and the corresponding pH gradient buffer solution is absorbed to the scale, and after mixing , put it in a...
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