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A method for identification and quantification of anaerobic iron-ammonia oxidation pathway in reservoir sediment

An anaerobic ferric ammonium oxidation and path identification technology, which is applied in the field of anaerobic ferric ammonium oxidation path identification and quantification in reservoir sediments, and can solve the problems of difficult quantitative evaluation of anaerobic ferric ammonium oxidation activity.

Active Publication Date: 2022-03-04
GUANGDONG UNIV OF TECH
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Problems solved by technology

[0009] The purpose of the present invention is to provide a method for identifying and quantifying the anaerobic iron ammonium oxidation pathway in the reservoir sediment, aiming at the problem that the existing anaerobic iron ammonium oxidation activity is difficult to quantitatively evaluate

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  • A method for identification and quantification of anaerobic iron-ammonia oxidation pathway in reservoir sediment
  • A method for identification and quantification of anaerobic iron-ammonia oxidation pathway in reservoir sediment
  • A method for identification and quantification of anaerobic iron-ammonia oxidation pathway in reservoir sediment

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Embodiment

[0093] This embodiment provides a 15 N-NH 4 + The method for marking and tracing the ammonia oxidation activity of anaerobic iron, the specific operation steps are as follows:

[0094] S1. Sediment pre-cultivation

[0095] Weigh 2-3g of sediment samples into ten 12mL Labco glass culture bottles, put two glass beads with a diameter of 3mm in each, divide the culture bottles into two groups on average, and mark them as control group and sample test group 1 respectively. In the control group, the needles were respectively filled with in-situ water aerated with high-purity nitrogen to ensure the same concentration of organic carbon sources and Fe(III) as in the natural sampling environment. while the test group is filled with pre-C 2 h 2 Aerated in situ water, which specifically inhibits heterotrophic denitrification and anammox activity, allowing accurate determination of anaerobic iron ammonium oxidation activity. Cover the original rubber stopper well, and there is no air...

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Abstract

The invention provides a method for identifying and quantifying the anaerobic iron ammonia oxidation path in reservoir bottom mud. The present invention adopts 15 N‑NH 4 + Mark and C 2 h 2 Inhibiting the method of heterotrophic denitrification and anammox activity, through the comparative analysis of the test group and the control group, the anaerobic iron ammonium oxidation reaction activity is calculated; the test group of other markers can also be added to further determine the anammox, Heterotrophic denitrification and co-denitrification activities. The method of the invention can not only measure the Feammox bacterial activity, but also can accurately distinguish the biological activity of other types of denitrification technologies, and provides a reliable evaluation method for systematic understanding and regulation of biological denitrification pathways.

Description

technical field [0001] The invention belongs to the field of surface drinking water treatment, and in particular relates to a method for identifying and quantifying anaerobic iron ammonium oxidation paths in reservoir bottom mud. Background technique [0002] A healthy cycle of nitrogen plays an important role in maintaining the stability and balance of Earth's ecosystems. However, human exploitation and utilization of mineral energy and excessive application of nitrogen-containing fertilizers have seriously accelerated the input of nitrogen into the environment, leading to serious nitrogen pollution in the atmosphere and water. Current traditional theory (Tian T, Yu H. Denitrification with non-organic electron donor for treating low C / N ratio wastewaters[J]. Bioresource Technology. 2020, 299: 122686. Zhang Z, Zhang Y, Chen Y. Recent advances in partial denitrificationin biological nitrogen removal:From enrichment to application[J].BioresourceTechnology.2020,298:122444.) be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/24C02F3/28C02F101/16
CPCC02F3/286G01N33/24C02F2101/16
Inventor 李宁江进许燕滨潘汉平关朝婷胡颖斌曹节
Owner GUANGDONG UNIV OF TECH
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