A kind of heterotrophic nitrifying aerobic denitrifying bacteria y15 and its application
An aerobic denitrifying bacteria and heterotrophic nitrification technology, applied in the field of environmental microorganisms, can solve problems such as limited practical application, and achieve the effects of high genetic stability, fast denitrification rate, and high genetic stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] Example 1 Isolation and identification of Pseudomonas putida Y15
[0035] 1. Isolation of Pseudomonas putida Y15
[0036] 1.1 Sample source
[0037] The samples were taken from the activated aerated sludge of a sewage treatment plant in Nanming District, Guiyang City.
[0038] 1.2 Enrichment, separation and purification of denitrification strains
[0039] Take 1mL activated sludge sample and inoculate it in the enrichment medium (formula: trisodium citrate 5.66g / L, MgSO 4 .7H 2 O0.02g / L, KH 2 PO 4 1.5g / L, Na 2 HPO 4 7.9g / L, KNO 3 0.84g / L, trace element solution 1mL / L, pH 7.41), enrichment culture for 72h at 28°C and 150rpm, repeated three times. Take 1mL of enriched culture solution dilution in 9mL of sterile normal saline, and dilute to 10 -1 . Dilute to 10 in turn -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 and 10 -7 isogradient. Under sterile conditions, take 10 -5 , 10 -6 and 10 -7 Each 200 μL of isogradient bacterial solution was evenly spread on the de...
Embodiment 2
[0056] Example 2 Antibiotic susceptibility test of Pseudomonas putida Y15
[0057] A single colony of Y15 was transferred into LB liquid medium and cultured at 37°C and 150rpm for 24h. Dilute the bacterial solution by 10 times, take 200 μL of the diluted solution and spread it evenly on the LB plate, stick three pieces of the same drug sensitive paper at equal distances on each plate, and place it in a 37°C constant temperature incubator for 24 hours, and count the drug susceptibility test results. . The results are shown in Table 2, Y15 is only resistant to erythromycin, sensitive to other antibiotics, and has high biosafety in sewage treatment.
[0058] Table 2 Y15 antibiotic susceptibility test results
[0059] Types of antibiotics Average antibacterial diameter (mm) Types of antibiotics Average antibacterial diameter (mm) norfloxacin 19.1±0.1 cifloxacin 23.1±0.2 Chloramphenicol 25.2±0.2 Amikacin 19.5±0.1 penicillin 26.1±0.1 Genta...
Embodiment 3
[0060] Example 3 Denitrogenation performance test of Pseudomonas putida Y15
[0061] The slanted seeds of Pseudomonas putida Y15 were transferred to 100 mL of LB liquid medium, and cultured at 30 °C and 150 rpm for 24 h to obtain seed liquid. The seed solution was taken out, centrifuged at 8000 rpm for 1 min, the supernatant was discarded, the bacterial cells were washed three times with sterilized PBS solution, and finally suspended in 10 mL of PBS solution to obtain bacterial solution. Take 0.5mL of this bacterial solution and transfer it to 500mL of NH 4 + -N, NO 3 - -N simulated sewage medium ((NH 4 ) 2 SO 4 0.72g / L; trisodium citrate dihydrate 3.50g / L; Vickers salt solution 50mL / L; trace element solution 1mL / L, pH 7.0. Wherein, the Vickers salt solution formula is: K 2 HPO 4 .3H 2 O 6.50g / L; MgSO 4 .7H 2 O 2.50g / L; NaCl 2.50g / L; FeSO 4 .7H 2 O 0.05g / L; MnSO 4 .H 2 O 0.04g / L. The formula of trace element solution is: EDTA 50g / L; ZnSO 4 2.20g / L; Ammonium M...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More