Method for simultaneously detecting multiple fat-soluble vitamins

A fat-soluble vitamin and vitamin technology, which can be used in measuring devices, instruments, scientific instruments, etc., can solve problems such as difficulties, large limitations, and increase testing costs, and achieve the goal of reducing testing costs, improving compliance, and reducing sample blood volume. Effect

Pending Publication Date: 2020-11-27
大连和合医学检验实验室有限公司
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, with the development of liquid chromatography and mass spectrometry, methods for detecting vitamins based on liquid chromatography and liquid chromatography-mass spectrometry have emerged. The Chinese patent, publication number: CN110763788A, discloses a method for quantitatively detecting five lipids in plasma. Kits and methods for soluble vitamins, however, the required blood collection volume is as high as 0.25mL, which is still difficult i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously detecting multiple fat-soluble vitamins
  • Method for simultaneously detecting multiple fat-soluble vitamins
  • Method for simultaneously detecting multiple fat-soluble vitamins

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1:

[0029] Step 1: Preparation of standard solution: preparation containing retinol (VA), 25-hydroxy vitamin D2, 25-hydroxy vitamin D3, α-tocopherol (VE) and the concentration of VK1 and VK2 are 1000, 40, 160, respectively 1mL of the mixed standard solution of, 11280, 100ng / mL, the solvent is chromatographic ethanol, label it as 1# tube, then prepare 8 test tubes, respectively label 2#-9#, add 200μL ethanol to each, and remove from 1# tube Take 200μL of the solution to the 2# tube, mix well and pipette 200μL to 3#, and mix until the 9# tube is completely mixed to obtain 9 standard mixed solutions of different concentrations.

[0030] Step 2: Preparation of internal standard solution: preparation containing d3-retinol, d6-α tocopherol, d3-25-hydroxyvitamin D2, d6-25-hydroxyvitamin D3, d7-VK1, d7-K1, d7-K2 Mixed internal standard solutions with concentrations of 3750, 5000, 25, 25, 833, 208ng / mL, and the solvent is chromatographic ethanol.

[0031] Step 3: Pre-treatme...

Example Embodiment

[0053] Example 2

[0054] Step 1: Collection of blood samples: venous blood from 64 healthy adults was collected using gel-promoting yellow cap blood collection tubes. The blood was centrifuged at 4000 rpm for 10 minutes to obtain serum. The 64 human serums were mixed uniformly to obtain mixed serum, which was stored in aliquots At -80°C. Take another 50mL of mixed serum, add vitamin A, E, D2, D3, K1, K2 solutions at the concentration of 1000, 20000, 100, 100, 10, 10ug / mL each 32.5, 32.5, 10.35, 20.5, 0.5, 0.5μL ( That is, 0.65, 13, 0.0207, 0.04106, 0.0001, 0.0001 ug of vitamin A, E, D2, D3, K1, K2) were added to each 1mL of mixed serum, mixed well to obtain the standard added serum, and stored in -80 ℃.

[0055] Step 2: Preparation of internal standard solution: preparation containing d3-retinol, d6-α tocopherol, d3-25-hydroxy vitamin D2, d6-25-hydroxy vitamin D3, d7-VK1, d7-K1, d7-K2 Mixed internal standard solutions with concentrations of 3750, 5000, 25, 25, 833, and 208ng / mL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses the technical field of fat-soluble vitamin detection and particularly relates to a method for simultaneously detecting multiple fat-soluble vitamins. The detection method comprises the following steps: step 1, fully and uniformly mixing 10-200 microlitres of sample plasma with a protein precipitation solution and a vitamin deuterated internal standard substance at 100-3000rpm for 1-5 min to obtain a solution A, and centrifuging the solution A at 0-8 DEG C at 200-12000 rpm to obtain a supernatant; and step 2, analyzing the fat-soluble vitamin of the supernatant by usinga liquid chromatograph-mass spectrometer, and calculating the content of the fat-soluble vitamin in the sample plasma by using an internal standard method. Compared with the prior art, the method hasthe advantages that more types of vitamins are detected, the sample can be enriched without the assistance of SLE, SPE and other materials, the detection cost is effectively reduced, the required blood volume is less than that of the existing method, the compliance of patients, especially infants, is better due to the reduction of the blood volume of the sample, and the method has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of detection of fat-soluble vitamins, and specifically relates to a method for simultaneously detecting six kinds of fat-soluble vitamins in blood plasma (clear). Background technique [0002] Vitamins, divided into fat-soluble vitamins and water-soluble vitamins, are a type of organic substances necessary to maintain human life activities, and are also important active substances to maintain human health. Vitamin deficiency or excess will lead to the occurrence of nutritional diseases; [0003] Vitamin A has various physiological functions such as promoting growth and reproduction, maintaining bone, epithelial tissue, vision and normal secretion of mucosal epithelium; vitamin D, also known as anti-rickets vitamin, is a sterol derivative, and the most important member of its family members is 25 hydroxy-vitamin D3 (cholecalciferol) and 25 hydroxy-vitamin D2 (ergocalciferol), both are fat-soluble vitamins, w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/32G01N30/16
CPCG01N30/02G01N30/06G01N30/34G01N30/32G01N30/16G01N2030/045G01N2030/062G01N2030/324
Inventor 晏嘉泽李世阳赵旭生徐恒宇隋文雯刘丽华刘韵姚玉涵万媛林琳
Owner 大连和合医学检验实验室有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap