A kind of enzyme-loaded silicon oxide millimeter sphere and its preparation method and application

An enzyme-loaded silicon oxide, enzyme-loaded silicon oxide technology, applied in the directions of silicon oxide, silicon dioxide, chemical instruments and methods, can solve problems such as increased fluid resistance, low mechanical strength, equipment blockage, etc. Simple, mild effects

Active Publication Date: 2021-11-19
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims at the disadvantages of low mechanical strength and small particle size of the enzyme-carrying catalytic material in the prior art, and filling into the reactor will easily lead to increased fluid resistance, equipment blockage, and enzyme loss or inactivation during the reaction process, and provides a High-strength enzyme-loaded silica millimeter sphere catalytic material that can be directly loaded into a fixed-bed reactor for enzyme-catalyzed reactions and preparation method thereof

Method used

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  • A kind of enzyme-loaded silicon oxide millimeter sphere and its preparation method and application
  • A kind of enzyme-loaded silicon oxide millimeter sphere and its preparation method and application
  • A kind of enzyme-loaded silicon oxide millimeter sphere and its preparation method and application

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Effect test

Embodiment 1

[0042] A method for preparing enzyme-loaded silicon oxide millimeter spheres, comprising the following steps:

[0043] Weigh 0.1g of hydrophilic mesoporous silica solid nanoparticles, ultrasonically disperse in 1.0mL of deionized water; then add 0.3mL of Candida antarctica lipase A solution with a concentration of 8mg / mL, and stir magnetically at 1200rpm for 5min Afterwards, the rotation speed was adjusted to 500 rpm, and the stirring was continued for 30 min to obtain the enzyme-loaded silicon oxide mixed solution.

[0044] Add the enzyme-containing silicon oxide mixture into a syringe with a needle inner diameter of 0.1 mm, and continuously drop the mixture at a flow rate of 0.5 mL / min on a superhydrophobic glass surface with a thickness of 1.0 mm spread on the surface of a glass plate through a syringe pump. On the powder of silicon oxide, the droplet rolls slowly under the action of a shaker to form a droplet millimeter sphere with a superhydrophobic silicon oxide shell. ...

Embodiment 2

[0047] A method for preparing enzyme-loaded silicon oxide millimeter spheres, comprising the following steps:

[0048] Weigh 0.2g of hydrophilic mesoporous silica solid nanoparticles, ultrasonically disperse in 1.5mL of deionized water; then add 0.5mL of Candida antarctica lipase B solution with a concentration of 8mg / mL, and stir magnetically at 1000rpm for 8min Afterwards, the rotation speed was adjusted to 400 rpm, and the stirring was continued for 40 min to obtain the enzyme-loaded silicon oxide mixed solution.

[0049]Add the enzyme-loaded silicon oxide mixture into a syringe with a needle inner diameter of 0.05 mm, and continuously drop the mixture at a flow rate of 0.3 mL / min on a superhydrophobic glass surface with a thickness of 2.0 mm spread on the surface of a glass plate through a syringe pump. On the powder of silicon oxide, the droplet rolls slowly under the action of a shaker to form a droplet millimeter sphere with a superhydrophobic silicon oxide shell.

[0...

Embodiment 3

[0052] A method for preparing enzyme-loaded silicon oxide millimeter spheres, comprising the following steps:

[0053] Weigh 0.2 g of hydrophilic mesoporous silica solid nanoparticles, ultrasonically disperse in 2.5 mL of deionized water; then add 0.5 mL of Candida antarctica lipase B solution with a concentration of 8 mg / mL, and stir magnetically at 600 rpm for 10 min Afterwards, the rotating speed was adjusted to 100 rpm, and the stirring was continued for 50 min to obtain the enzyme-loaded silicon oxide mixed solution.

[0054] Add the enzyme-loaded silicon oxide mixture into a syringe with a needle inner diameter of 0.15 mm, and continuously drop the mixture at a flow rate of 0.05 mL / min on a superhydrophobic oxide surface with a thickness of 1.0 mm on the surface of a glass plate through a syringe pump. On the powder of silicon, the droplet rolls slowly under the action of a shaker to form a droplet millimeter sphere with a superhydrophobic silicon oxide shell.

[0055] ...

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Abstract

The invention belongs to the field of preparation of catalytic materials, and in particular relates to an enzyme-loaded silicon oxide millimeter sphere, a preparation method and application thereof. The particle size of the enzyme-loaded silica millimeter balls is 0.90-5.0mm, the mechanical strength is as high as 3.0-25N, and the enzyme content is 4.8-80mg / g. The main preparation process is to disperse hydrophilic mesoporous silica solid nanoparticles Add the enzyme solution to the deionized water to form a mixed solution, then drop the mixed solution continuously on the superhydrophobic silica powder, roll slowly to form a droplet millimeter ball with a silicon oxide shell; finally collect the droplet millimeter ball slowly Dry to obtain high-strength enzyme-loaded silica millimeter spheres. The enzyme-loaded silica millimeter spheres prepared by the method can be directly loaded into a fixed-bed reactor to carry out enzyme-catalyzed reaction, and the enzyme-catalyzed activity remains basically unchanged for 30 to 60 days of continuous reaction.

Description

technical field [0001] The invention belongs to the field of preparation of catalytic materials, and in particular relates to an enzyme-loaded silicon oxide millimeter sphere, a preparation method and application thereof. Background technique [0002] Due to the advantages of easy control, high catalytic efficiency, and easy recycling and reuse, immobilized enzymes are widely used in the fields of pharmaceuticals, food, wastewater treatment, and daily chemical industries. At present, the main methods of immobilizing enzymes include physical methods (adsorption and embedding) and chemical methods (covalent and cross-linking). The advantage of immobilizing enzymes by physical methods is that the conditions are mild, and the structure and activity of enzymes can be preserved to the greatest extent; the disadvantage is that the binding force between enzymes and carriers is weak, and enzyme molecules are easy to fall off; the advantage of immobilizing enzymes by chemical methods ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J21/08
CPCB01J21/08B01J35/08B01J35/1023B01J35/1061C01B33/12
Inventor 杨恒权郭晓苗刘宪
Owner SHANXI UNIV
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