Bletilla striata seed low-temperature storage device and method

Abstract

The invention discloses a bletilla striata seed low-temperature storage device and method. The device comprises a liquid nitrogen tank; a base is arranged at the bottom of the interior of the liquid nitrogen tank; a plurality of stand columns are vertically arranged on the edge of the surface of the base; a storage mechanism is installed at the top of the base; the storage mechanism comprises trays and screw rods, and the multiple trays are sequentially stacked on the base; positioning holes are formed in the edges of the trays; the screw rods sequentially penetrate through the trays in a threaded manner from top to bottom; and the top of the liquid nitrogen tank is rotationally connected with a sealing cover, and an adjusting sliding rod penetrates through the center of the sealing coverin a sliding mode. The method comprises the steps of S1, sterilizing seeds; S2, preparing a matrix solution; S3, filling the seeds; and S4, refrigerating the seeds. According to the invention, duringrefrigeration, the cell activity of the seeds is reduced, so that the seeds can be stored for a long time; and matrix layers wrapping the outer walls of the seeds can be solidified into solid, and thematrix layers can play a role in external protection during refrigeration, so that the seeds are prevented from directly making contact with liquid nitrogen to be frostbitten, and the survival rate of the seeds is increased.

Description

technical field [0001] The invention belongs to the technical field of bletilla seeds, in particular to a low-temperature storage device and method for bletilla seeds. Background technique [0002] White and the surface is off-white or yellow-white, with several circles of concentric links and brown dot-like fibrous root scars, with raised stem scars on the top and traces of connecting another tuber on the bottom; the texture is hard, not easy to break, and the cross-section is off-white; it has the properties of astringent hemostasis, The drug effect of reducing swelling and promoting muscle growth; [0003] When there are too many seeds to be picked and cannot be planted immediately, it is necessary to refrigerate and store the seeds. There are two ways to refrigerate them. The first is to store them in the refrigerator. However, the temperature of the refrigerator is not low enough to achieve good preservation. Effect; the second is liquid nitrogen storage, the storage t...

AI Technical Summary

Problems solved by technology

[0003] When there are too many seeds to be picked and cannot be planted immediately, it is necessary to refrigerate and store the seeds. There are two ways to refrigerate them. The first is to store them in the refrigerator. However, the temperature of the refrigerator is not low enough to achieve good preservation. Effect; the second is liquid nitrogen storage, the storage temperature of liquid nitrogen is very low, and the seeds are easy to frostbite

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