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Preparation method of plant inducer

A plant inducer and physical method technology, applied in the field of agricultural biology, can solve problems such as insufficient control of plant diseases and restrictions on agricultural production

Active Publication Date: 2020-12-18
山东元泰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] An important issue worldwide is the insufficient control of plant diseases, which limits the annual agricultural production worldwide.

Method used

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  • Preparation method of plant inducer
  • Preparation method of plant inducer
  • Preparation method of plant inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of plant inducer, comprising the following steps:

[0040] Step 1: Resuscitate the bacteria stored in glycerin, shake in the vial at 37°C and 100rpm in the dark for 1 day, and cultivate the strains of Bacillus pumilus and Bacillus arseni;

[0041] Step 2: Inoculate the culture in a fermenter and ferment at 37°C for 48 hours;

[0042] Step 3: Treat the culture filtrate by physical method. The culture filtrate is treated by physical method, respectively in liquid nitrogen for 45 seconds, in hot water for 45 seconds, and repeat three times; after drying, the inducer is obtained.

Embodiment 2

[0044] A preparation method of plant inducer, comprising the following steps:

[0045] Step 1: Resuscitate the bacteria stored in glycerin, shake in the vial at 37°C and 100rpm in the dark for 1 day, and cultivate the strains of Bacillus pumilus and Bacillus arseni;

[0046] Step 2: Inoculate the culture in a fermenter and ferment at 37°C for 48 hours;

[0047] Step 3: Treat the culture filtrate by physical method. The culture filtrate is treated by physical method, respectively in liquid nitrogen for 40 seconds, in hot water for 50 seconds, and repeated four times; after drying, the inducer is obtained.

Embodiment 3

[0049] A preparation method of plant inducer, comprising the following steps:

[0050] Step 1: Resuscitate the bacteria stored in glycerin, shake in the vial at 37°C and 100rpm in the dark for 1 day, and cultivate the strains of Bacillus pumilus and Bacillus arseni;

[0051] Step 2: Inoculate the culture in a fermenter and ferment at 37°C for 48 hours;

[0052] Step 3: Treat the culture filtrate by physical method. The culture filtrate is treated by physical method, respectively in liquid nitrogen for 50 seconds, in hot water for 40 seconds, and repeated five times; after drying, the inducer is obtained.

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Abstract

The invention discloses a preparation method of a plant inducer, and relates to the technical field of agricultural biology. The preparation method comprises the following steps: step 1, culturing strains of bacillus pumilus and bacillus aryabhattai; step 2, carrying out fermentation after inoculation; and step 3, treating the culture filtrate by a physical method, and drying to obtain the inducer. The invention discloses a method for inducing plant stress resistance for a long time by activating genes related to plant defense. The present invention prevents and treats plant diseases caused bypathogens by utilizing specific molecules from Bacillus pumilus and Bacillus aryabhattai compounds.

Description

technical field [0001] The invention relates to the technical field of agricultural biotechnology, in particular to a preparation method of a plant inducer. Background technique [0002] In recent years, food security determined by the balance of global food supply and demand has increasingly become a topic of common concern. The continuous growth of population has increased the demand for food. However, the continued decline in the area of ​​agricultural land has led to a decline in food production. While the reasons for the decline in yields are varied, pests and diseases are a significant contributor to crop yield declines worldwide. Crop losses due to pathogen infection range from 20% to 40%. Crop disease detection and prevention can minimize the risk of pathogenic bacteria during crop growth, harvesting and post-harvest processing, and maximize productivity and ensure sustainable agricultural development. This illustrates the importance of understanding plant diseas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22A01P21/00
CPCA01N63/22
Inventor 奥兰多·鲍里斯·伊达尔戈徐洪利高祥友
Owner 山东元泰生物工程有限公司