Tibetan ganoderma lucidum polysaccharide GLP-1 with antioxidant effect as well as preparation method and application thereof

A technology of GLP-1 and CGLP-1, which is applied in the research field of Ganoderma lucidum polysaccharides, can solve the problems of Tibetan Ganoderma lucidum polysaccharides with few reports on anti-oxidative biological activity, Tibetan Ganoderma lucidum polysaccharides and less

Active Publication Date: 2020-12-18
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the existing technology, there are few research results on the new components of Tibetan Ganoderma lucidum polysaccharides, and there are few reports on the antioxidant biological activity of Tibetan Ganoderma lucidum polysaccharides

Method used

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  • Tibetan ganoderma lucidum polysaccharide GLP-1 with antioxidant effect as well as preparation method and application thereof
  • Tibetan ganoderma lucidum polysaccharide GLP-1 with antioxidant effect as well as preparation method and application thereof
  • Tibetan ganoderma lucidum polysaccharide GLP-1 with antioxidant effect as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1) Preparation of Tibetan Ganoderma lucidum polysaccharide GLP-1

[0060] Add 95% ethanol to Tibetan Ganoderma lucidum powder, extract according to the ratio of material to liquid 1:20, extraction temperature 75°C for 2 hours, repeat the operation once, and finally dry Tibetan Ganoderma lucidum to obtain Tibetan Ganoderma lucidum defatted powder; add water to Tibetan Ganoderma lucidum defatted powder (Tibetan Ganoderma lucidum) Ganoderma lucidum degreased powder and water were extracted at 90°C for 2 hours according to the ratio of solid to liquid (1:20), filtered to obtain the water extract, and the operation was repeated twice, and then the water extract was concentrated under reduced pressure at 60°C to obtain concentrated liquid;

[0061] Add 4 times the volume of absolute ethanol to the concentrated solution, let stand at 4°C for 16 hours, precipitate the polysaccharide, then centrifuge at 4°C to collect the precipitate, centrifuge at 6000rpm, centrifuge for 10min,...

Embodiment 2

[0093] 1) The scavenging ability of Tibetan ganoderma lucidum polysaccharide GLP-1 on ABTS free radicals

[0094] ABTS · + It was used by reacting 7 mM ABTS stock solution with 2.45 mM potassium persulfate (final concentration) and allowing the mixture to stand in the dark at room temperature for 16 hours. Dilute ABTS with PBS + Solution, mixed with an equal amount of ultrapure water at 734nm, the absorbance value is 0.70 (±0.05). Mix 100 μL GLP-1 solution with 100 μL ABTS· + The solutions were mixed, and placed in the dark at 30° C. for 6 minutes, and measured at 734 nm using a microplate reader, and Vc was used as a positive control. The result is as Figure 13 As shown in A, it can be seen that: GLP-1 can scavenge ABTS free radicals, and its IC 50 It is 0.56mg / mL, but the clearance ability is weaker than that of Vc.

[0095] 2) The scavenging ability of Ganoderma lucidum polysaccharide GLP-1 to hydroxyl free radicals

[0096] Mix 50 μL of ferrous sulfate (1.5mM) and ...

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Abstract

The invention discloses Tibetan ganoderma lucidum polysaccharide GLP-1 with antioxidant efficacy, a preparation method and application; wherein the ganoderma lucidum polysaccharide GLP-1 is extractedfrom Tibetan ganoderma lucidum powder, the polysaccharide content is 73.36%, the uronic acid content is 2.27%, the weight-average molecular weight is 6.31 kDa, and the protein content is extremely low. The Tibetan ganoderma lucidum polysaccharide GLP-1 is separated and prepared from Tibetan ganoderma lucidum for the first time, wherein the GLP-1 comprises mannose, glucose, galactose, xylose, arabinose and the like, has characteristic absorption peaks of OH, CH, COH, alpha isomeric pyranose and alpha, beta glucosidic bonds, does not have a triple helix structure, and is good in thermal stability; besides, the obtained polysaccharide GLP-1 has chemical antioxidant activity, can protect mouse fibroblasts NIH3T3 from oxidative damage caused by tert-butyl hydroperoxide induction, and has good edible and medicinal values in the aspect of antioxidant activity.

Description

technical field [0001] The invention relates to the research field of Ganoderma lucidum polysaccharides, in particular to Tibetan Ganoderma lucidum polysaccharide GLP-1 with antioxidant effect, a preparation method and application. Background technique [0002] Ganoderma lucidum Karst (Ganoderma lucidum Karst), also known as Lin Zhongling and Qiongzhen, is the fruiting body of the polyporaceae fungus Ganoderma lucidum, belonging to Eumycota, Basidiomycotina, Hymenomycetes, Polypore Aphyllophorales, Gamodermataceae, Ganoderma. Ganoderma lucidum has good names such as "auspicious grass", "immortal grass", "shenzhi" and "linzhongling". It is a medicinal and edible fungus of the genus Ganoderma. Ganoderma lucidum is a commonly used traditional Chinese medicine in my country. It contains polysaccharides, triterpenes and sterols and other active substances. Many ancient pharmacy monographs such as "Shen Nong's Materia Medica" and "Compendium of Materia Medica" have recorded a va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06A61K31/715A23L33/125
CPCC08B37/0003C08B37/006A61K31/715A61P39/06A23L33/125A23V2002/00A23V2200/02A23V2250/208A23V2250/51
Inventor 高雄吴清平谢意珍穆静静胡惠萍莫伟鹏
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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