Real-time fluorescence quantitative acquisition signal filtering and amplifying circuit
A filter amplifying circuit, real-time fluorescence quantitative technology, applied in the direction of amplification control, amplifier combination, improving amplifiers to reduce noise effects, etc. Inaccurate signal acquisition and other problems, to achieve the effect of facilitating data analysis, filtering out useless signals, and high acquisition efficiency
Pending Publication Date: 2020-12-18
BIOTEKE CORP (WUXI) CO LTD
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[0006] Purpose of the invention: In order to solve the problems of inaccurate fluorescence signal acquisition, low fluorescence signal acquisition efficiency, and inability to adjust the magnification and filtering effect according to the strength of the fluorescence signal in the prior art, a real-time fluorescence quantitative acquisition signal filter amplification is proposed. circuit
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[0030] The technical solution of the present invention will be further described in conjunction with the accompanying drawings and embodiments.
[0031] Such as figure 1 As shown, using the filter amplifier circuit of the present invention, the processing flow of the fluorescent signal includes: firstly, the fluorescent signal is collected through the silicon photoelectric sensor circuit S1, and the fluorescent signal is converted into a current signal, and the current signal is sequentially passed through the IV conversion pre-amplification circuit And the second-order voltage-controlled low-pass filter circuit to eliminate background and circuit noise; then through the multi-stage filter amplifier circuit, automatically adjust the signal amplification factor for the signal entering the multi-stage filter amplifier circuit; finally, through the high-precision multi-channel ADC modulus The conversion circuit converts the analog signal into a digital signal, so that the digital...
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The invention provides a real-time fluorescence quantitative acquisition signal filtering and amplifying circuit, which comprises a silicon photoelectric sensor, a multi-order filtering and amplifyingcircuit and an ADC analog-to-digital conversion circuit, and an output digital signal can reflect a specific DNA sequence and perform quantitative analysis; the multi-order filtering and amplifying circuit comprises a plurality of filtering and amplifying circuits, the output end of each filtering and amplifying circuit is connected with the input end of the next filtering and amplifying circuit,the output end of the next filtering and amplifying circuit is connected with the input end of the next filtering and amplifying circuit, and the like, so that the multi-order filtering and amplifying circuit is formed; and the output end of each filtering and amplifying circuit is connected to the ADC analog-to-digital conversion circuit, by means of the filtering and amplifying circuits, the amplification factor and the filtering effect can be automatically adjusted according to the intensity of fluorescence signals in different stages of DNA amplification, and subsequent data analysis is facilitated.
Description
technical field [0001] The invention belongs to the technical field of electronic circuits, and in particular relates to a real-time fluorescence quantitative acquisition signal filter amplifier circuit. Background technique [0002] Real-time fluorescent quantitative PCR (Quantitative Real-time PCR) is a DNA amplification reaction that uses fluorescent chemicals to measure the total amount of products after each polymerase chain reaction (PCR) cycle, and is tested by internal reference or external reference method. A method for the quantitative analysis of specific DNA sequences in a sample. Real-time PCR is a real-time detection of the PCR process through fluorescent signals during the PCR amplification process. [0003] The basic principle of PCR technology is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. PCR consists of three basic reaction steps: denaturation...
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IPC IPC(8): H03F1/26H03F3/68H03M1/12H03G3/30
CPCH03F1/26H03F3/68H03G3/3084H03G3/3089H03M1/1205
Inventor 周志图
Owner BIOTEKE CORP (WUXI) CO LTD