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Semi-quantitative method for fecal occult blood detection

A semi-quantitative, occult blood technology, applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of reducing efficiency and detection accuracy, inability to judge the amount of gastrointestinal bleeding, and increasing the manual workload, so as to reduce the artificial complexity. The work of checking, solving the defects of fecal occult blood detection, and reducing the effect of manual workload

Inactive Publication Date: 2020-12-22
上海宏勃生物科技发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Most clinical in vitro diagnostic equipment for the detection of fecal occult blood in fecal samples is colloidal gold immunoassay, which can only be used for clinical fecal occult blood qualitative, but cannot judge the amount of gastrointestinal bleeding, and the interpretation of the results is subjective and closely related to the experience of the examiner relevant
Qualitative test results can only reflect whether there is bleeding, and the amount of bleeding can only be obtained through quantitative or semi-quantitative analysis of fecal occult blood, which may even increase manual workload and greatly reduce the efficiency and accuracy of detection

Method used

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  • Semi-quantitative method for fecal occult blood detection
  • Semi-quantitative method for fecal occult blood detection
  • Semi-quantitative method for fecal occult blood detection

Examples

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Effect test

Embodiment 1

[0026] Reagents: colloidal gold test card, hemoglobin negative quality control, hemoglobin low value quality control, hemoglobin high value quality control, 3,3,5,5-tetramethylbenzidine, hydrogen peroxide solution.

[0027] Experimental method: At room temperature, divide the same batch of colloidal gold test cards into three groups, which are hemoglobin negative quality control products, hemoglobin low-value quality control products, and hemoglobin high-value quality control products. The experiment is repeated three times for each group. Add 100 μl each of hemoglobin negative quality control, hemoglobin low-value quality control and hemoglobin high-value quality control to the sample hole of the colloidal gold test card, observe the qualitative negative and positive results after reacting for 60 seconds, and then drop them successively 50 μL of 3,3,5,5-tetramethylbenzidine indicator solution with a mass concentration of 0.3 g / L, and then drop 50 μL of a 3% hydrogen peroxide s...

Embodiment 2

[0030] Reagents: colloidal gold test card, low-value hemoglobin quality control, median hemoglobin quality control, high-hemoglobin quality control, 3,3,5,5-tetramethylbenzidine, hydrogen peroxide solution.

[0031] Experimental method: At room temperature, divide the same batch of colloidal gold test cards into three groups, namely low-value hemoglobin quality control products, medium-value hemoglobin quality control products, and high-value hemoglobin quality control products. The experiment was repeated three times for each group. First, Add 100 μl each of the hemoglobin low-value quality control, hemoglobin median hemoglobin quality control, and hemoglobin high-value quality control to the sample hole of the colloidal gold test card, and observe the qualitative negative and positive results after reacting for 60 seconds, and then sequentially Add dropwise 50μL of 3,3,5,5-tetramethylbenzidine indicator solution with a mass concentration of 0.3g / L, and then dropwise add 50μL ...

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Abstract

The invention provides a fecal occult blood detection semi-quantitative method which comprises the following steps: dropwise adding a diluted sample into a sample application hole in a colloidal golddetection card by virtue of a sample application device, conducting photographing and reading a qualitative result on a display by virtue of a reading instrument after qualitative negative and positive are initially shown; reading a qualitative result, dropwise adding a certain amount of 3,3,5,5-tetramethyl benzidine indicating solution into the same sample application hole, and dropwise adding acertain amount of hydrogen peroxide solution; and after dropwise adding the 3,3,5,5-tetramethyl benzidine indicating solution and the hydrogen peroxide solution for a period of reaction time, displaying a semi-quantitative result by a colloidal gold detection card, and conducting photographing again to read the semi-quantitative result. According to the invention, a semi-quantitative result of fecal occult blood detection is increased, manual operation is reduced, and manual workload is reduced, so that the defect of fecal occult blood detection in clinical examination is overcome, the work ofmanual reexamination is greatly reduced, and the accuracy of a detection result is ensured.

Description

technical field [0001] The technical field of clinical stool detection of the present invention, in particular relates to a semi-quantitative method for detection of fecal occult blood. Background technique [0002] Most clinical in vitro diagnostic equipment for the detection of fecal occult blood in fecal samples is colloidal gold immunoassay, which can only be used for clinical fecal occult blood qualitative, but cannot judge the amount of gastrointestinal bleeding, and the interpretation of the results is subjective and closely related to the experience of the examiner relevant. Qualitative test results can only reflect whether there is bleeding, and the amount of bleeding can only be obtained through quantitative or semi-quantitative analysis of fecal occult blood, which may even increase the workload of humans and greatly reduce the efficiency and accuracy of detection. [0003] Therefore, the present invention provides a semi-quantitative method for detecting fecal o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72
CPCG01N33/725
Inventor 张嘉桐赵国军刘律文周华明李昂陈健李文倩张帅
Owner 上海宏勃生物科技发展有限公司