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Application of MIT and/or DIT as thyroid cancer markers and kit

A technology of thyroid cancer and markers, which is applied in the field of bioanalysis and medicine, can solve the problems of diagnosis, failure of cancer, and obvious effect of cancer diagnosis, achieving high specificity and effectiveness, low tissue sample consumption, and simple and convenient pretreatment Effect

Active Publication Date: 2021-01-01
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The palpation method cannot identify the real cancer incidence; the imaging examination method can only obtain early or advanced cancer images and cannot diagnose the cancer; There are certain difficulties in typing, and it is only effective for the diagnosis of most advanced cancers
Commonly used thyroid hormone test indicators (such as T3, T4) in the blood cannot reflect the incidence of cancer
Generally speaking, there are many methods for the diagnosis of thyroid cancer at present, but each method has certain limitations, making the diagnosis of thyroid cancer more difficult

Method used

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  • Application of MIT and/or DIT as thyroid cancer markers and kit
  • Application of MIT and/or DIT as thyroid cancer markers and kit
  • Application of MIT and/or DIT as thyroid cancer markers and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Determination of Tumor Marker Concentrations in Normal Thyroid Cells and Thyroid Cancer Cells

[0039] 1. Instruments and reagents:

[0040] Ultra-high performance liquid chromatography tandem mass spectrometry (Waters Company), including ACQUITY ultra-high performance liquid phase system, Xevo TQ-XS quadrupole mass spectrometer; high-speed refrigerated centrifuge; analytical balance; nitrogen blowing instrument; vortex shaker.

[0041] Methanol (LC / MS grade), Milli-Q water.

[0042]2. Pretreatment of normal thyroid cells and thyroid cancer cells

[0043] (1) Accurately weigh 0.1-1 mg of normal thyroid cells and thyroid cancer cells to be tested, add 50 μl of methanol internal standard solution, homogenate and break up the tissue samples, mix them and place them in a low temperature environment below 0°C, take them out after 15 minutes ; Greater than 8000g centrifugal force, centrifuge for 5-15 minutes. Add 50 μl of methanol to the precipitate, extract, ce...

Embodiment 2

[0054] Example 2: Determination of Tumor Marker Concentrations in Normal Thyroid Tissue and Thyroid Cancer Tissue

[0055] 1. Instruments and reagents:

[0056] With embodiment 1.

[0057] 2. Pretreatment of normal thyroid tissue and thyroid cancer tissue

[0058] (1) Accurately weigh 0.1-1 mg of the normal thyroid tissue and thyroid cancer tissue samples to be tested, add 50 μl of methanol internal standard solution, homogenize and break the tissue samples, mix them and place them in a low temperature environment below 0°C, take them out after 15 minutes ; Greater than 8000g centrifugal force, centrifuge for 5-15 minutes. Add 50 μl of methanol to the precipitate, extract, centrifuge, and collect the supernatant, repeat twice, and mix the obtained supernatant to obtain a tumor marker extract.

[0059] (2) Derivatization reaction: Take 30 μl of the derivatization reaction buffer solution in a 2 mL brown bottle, add the tumor marker extract extracted in step (1), add 20 μl, 3...

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Abstract

The invention discloses application of MIT and / or DIT as thyroid cancer markers and a kit. The MIT and the DIT are synthetic precursor substances of thyroid hormone T4, are stably and constantly expressed in thyroid tissue, and have high specificity and effectiveness. According to the invention, conditions that expression quantities of the MIT and the DIT are quite remarkably different in thyroidcancer tissue and thyroid normal tissue, and the expression quantities of the MIT and the DIT in the thyroid cancer tissue are 100-200 times lower than of the expression quantities of the MIT and theDIT in the normal tissue are found for the first time. Based on this, the MIT and the DIT can be used as biomarkers for thyroid cancer detection, corresponding analysis methods can be used for preparing a thyroid cancer detection kit, and a new technical means is provided for thyroid cancer detection, diagnosis, treatment and prognosis evaluation. According to the kit, a thyroid cancer sample collected by a puncture needle can be accurately analyzed, and the purpose of ultra-efficient and high-sensitivity detection of the kit is achieved.

Description

technical field [0001] The invention relates to the fields of bioanalysis technology and medicine, in particular to a biomarker specific for thyroid cancer, its application and a cancer detection kit. Background technique [0002] Thyroid cancer is the most common clinical endocrine malignancy. In recent years, the clinical incidence has shown a significant increase, and the trend is getting younger and younger. It has been confirmed in current medical research that this disease will also be accompanied by a spectrum of diseases. Changes in the disease lead to a gradual increase in clinical morbidity. At present, the commonly used clinical techniques for diagnosing thyroid cancer include: palpation, imaging and cytology, etc., but there are still problems such as low one-time diagnosis rate and the need for multiple diagnoses. The palpation method cannot identify the real cancer incidence; the imaging examination method can only obtain early or advanced cancer images and ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/04G01N30/14G01N30/72G01N30/86G01N30/88
CPCG01N30/02G01N30/06G01N30/04G01N30/14G01N30/72G01N30/8634G01N30/88G01N2030/067G01N2030/062G01N2030/045G01N2030/8822
Inventor 万祎崔洪洋
Owner PEKING UNIV
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