A heterogeneous data-independent proteomics mass spectrometry system and method

Abstract

The invention discloses a heterogeneous data independent proteomics mass spectrometry analysis system and method. The system includes a local client and a cloud high-performance server. The method includes the following steps: (1) a local client reads local heterogeneous data-independent proteomics mass spectrometry data, calls a cloud high-performance server, and obtains a data interpreter; (2) the local client completes the data locally After interpretation, spectrum extraction, and pseudo-peptide generation, the peptide spectrum data, pseudo-peptide and target detection peptide are submitted to the high-performance server; (3) the high-performance server, according to the local client Data analysis is performed on the provided peptide spectrum data, pseudo-peptides, and target detection peptides, and the calculated proteomics analysis results are returned to the local client. The invention can take into account the privacy of the original data-independent proteomics mass spectrometry data and the powerful computing power.

Description

technical field [0001] The invention belongs to the field of proteomics, and more particularly, relates to a heterogeneous library file data-independent proteomics mass spectrometry analysis system and method. Background technique [0002] Traditional proteomics employs a data-dependent acquisition (DDA) strategy in which protein samples are digested into peptide fragments, ionized, and analyzed by mass spectrometry. In full scan mass spectra, peptide signals above noise are selectively cleaved, resulting in random (MS / MS) mass spectra that can be matched to the spectra in the database. Although this method is very powerful, it randomly samples peptides for cleavage, always favoring those peaks with the strongest signal. Therefore, quantification of low-abundance peptides remains a challenge. [0003] Since then, a directional analysis technique, Selected Reaction Monitoring (SRM), has been developed, in which mass spectrometers can detect specific peptides very sensitivel...

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Problems solved by technology

[0006] In view of the above defects or improvement needs of the prior art, the present invention provides a heterogeneous data-independent proteomics mass spectrometry system and method, the purpose of which is to realize scalable compatible complex The data-independent proteomics mass spectrometry data format, at the same time, under the premise of ensuring data privacy and security, shortens the data analysis time and reduces the local requirements for computing power, thus solving the problem that the existing analysis software cannot be scalable and compatible Continuously updated data formats, high requirements for local computing power, long analysis time, risk of data privacy leakage, and technical issues that are not conducive to commercial promotion

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