A Molecular Marker of Copy Number Variation on Pig Chromosome 11 Affecting Pig Backfat Thickness and Its Application

A technology for copy number variation and pig backfat thickness, which is applied in the field of molecular biotechnology and molecular markers, can solve the problems of phenotypic variation and large influence on gene expression, and achieve the effect of speeding up the breeding process, improving the carcass structure and reducing the breeding loss.

Active Publication Date: 2022-08-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with SNP, CNV has a greater impact on gene expression, leading to phenotypic variation by changing gene structure, gene regulation and exposing recessive harmful genes

Method used

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  • A Molecular Marker of Copy Number Variation on Pig Chromosome 11 Affecting Pig Backfat Thickness and Its Application
  • A Molecular Marker of Copy Number Variation on Pig Chromosome 11 Affecting Pig Backfat Thickness and Its Application
  • A Molecular Marker of Copy Number Variation on Pig Chromosome 11 Affecting Pig Backfat Thickness and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Experimental animals

[0045] The experimental pig herd group used in the present invention is the pure-bred American Duroc pigs of the breeding pig branch of Wen's Food Group Co., Ltd., which is the core group of the breeding pig branch, and the herd system pedigree is recorded in detail. In this experiment, a total of 3770 American Duroc breeding pigs in this resource group were selected, and the pigs were kept free to eat and drink during the feeding process. The whole feeding method and feeding conditions were always consistent, which was a conventional method.

[0046] (2) Sample collection

[0047] The above-mentioned piglet docked tail and ear tissues were collected, soaked in 75% ethanol solution by volume, and stored in a -20°C refrigerator for later use.

[0048] (3) Types of 50K SNPs in the whole pig genome

[0049] The whole genome DNA was extracted from the ear tissue or tail tissue collected from each individual of the 3770 Duroc breeding pigs select...

Embodiment 2

[0059] Example 2 Amplification and sequencing of target DNA sequences

[0060] (1) Primer design

[0061] The specific DNA sequence (SEQ ID NO: 1) located in the sequence of 77144460bp-78780052bp on chromosome 11 of pig was downloaded from the NCBI website (http: / / www.ncbi.nlm.nih.gov / ) as the reference sequence, and qPCR primers (primer pair P1) were designed using primer design software primer premier 6.0. At the same time, using the porcine GCG gene sequence published by NCBI as the reference sequence, the qPCR primers (primer pair P2) were designed by the same method. The DNA sequences of the designed primers are shown below.

[0062] Primer pair P1 is:

[0063] Upstream primer F1: 5'-CTCCACTTGTGTAAGCCGCTCATC-3',

[0064] Downstream primer R1: 5'-CACCTTGCTGGAAGTCGCTGTC-3';

[0065] Primer pair P2 is:

[0066] Upstream primer F2: 5'-TGCAGAACTACAGGGCTCAGGAC-3',

[0067] Downstream primer R2: 5'-GTGGAACAGGGCAGGTGGAAAG-3';

[0068] (2) qPCR amplification

[0069] Add ...

Embodiment 3

[0080] Example 3 Copy number type effect analysis of copy number variation molecular markers

[0081] According to Table 1, the effect of the copy number gain type in the CNV region was significantly lower than that of the copy number deletion type by 0.9 mm. The invention provides a copy number variation molecular marker that can significantly reduce the back fat thickness of Duroc pigs, and uses the copy number variation molecular marker to carry out marker-assisted selection to gradually eliminate the copy number deletion type pig individuals in the population and improve the copy number increase type and The normal frequency can greatly speed up the breeding process of Duroc breeding pigs' lean meat rate, improve the carcass performance of pigs, and produce more high-quality lean-type commercial pigs to meet people's demand for high-quality pork, thus driving the The increase in pork sales will bring huge economic benefits to the enterprise.

[0082] The invention provide...

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Abstract

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to a copy number variation molecular marker on pig chromosome 11 that affects pig backfat thickness and its application. The copy number variation region of the copy number variation molecular marker that affects pig backfat thickness on the pig chromosome 11 corresponds to the fragment located in the 77144460bp-78780052bp interval on the chromosome 11 of the reference sequence of the international pig genome version 11.1, and is prone to occur in this region Copy number variation and association with pig backfat. By optimizing the dominant copy number type of the copy number variation molecular marker, the invention can increase the frequency of the dominant copy number type from generation to generation, reduce pig backfat thickness, speed up the progress of pig genetic improvement, and effectively improve the economic benefit of breeding pigs.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to a copy number variation molecular marker on pig chromosome 11 that affects pig backfat thickness and its application. Background technique [0002] Pigs are one of the most important livestock, providing a large amount of animal protein for humans. After the reform and opening up, lean pigs have become the mainstream of my country's pork consumption market. Therefore, breeding high-quality lean pigs has become the focus of breeding work. Subcutaneous fat is the largest part of pig body fat deposition, of which backfat is the result of subcutaneous fat deposition. In addition, backfat thickness is an important indicator of lean meat percentage in production, which is closely related to carcass performance, feed utilization efficiency and fecundity. Therefore, the improvement of backfat thickness is an important reference factor f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/156C12Q2600/124C12Q2531/113C12Q2545/101C12Q2537/16Y02P60/87
Inventor 杨杰吴珍芳阮栋林郑恩琴丁荣荣邱益彬庄站伟吴杰顾婷洪林君徐铮黄思秀
Owner SOUTH CHINA AGRI UNIV
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