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SNP molecular markers and applications related to lean meat rate, eye muscle area and eye muscle thickness on pig chromosome 6

A technology of eye muscle area and molecular markers, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of limiting the application of molecular markers for complex traits, large confidence regions, etc., to increase core competitiveness , increase the lean meat rate, and speed up the breeding process

Active Publication Date: 2022-07-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the candidate gene method has the advantages of simple method and convenient operation, it can only target genes with known biological functions; while the confidence region of the candidate gene identified by the QTL mapping method is also large, which greatly limits the ability of complex trait molecules Application of markers in livestock genetics and breeding

Method used

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  • SNP molecular markers and applications related to lean meat rate, eye muscle area and eye muscle thickness on pig chromosome 6
  • SNP molecular markers and applications related to lean meat rate, eye muscle area and eye muscle thickness on pig chromosome 6
  • SNP molecular markers and applications related to lean meat rate, eye muscle area and eye muscle thickness on pig chromosome 6

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Experimental animals

[0037] The experimental pig herd group used in the present invention is 3916 purebred American Duroc heads of the breeding pig branch of Wen's Food Group Co., Ltd., which is the core group of the breeding pig branch.

[0038] In this experiment, a total of Sino-American Duroc breeding pigs were selected from this resource group. The pigs had free access to food and water, and the whole feeding method and feeding conditions were always consistent, which was a conventional method.

[0039] (2) Sample collection

[0040] The above-mentioned piglet docked tail and ear tissues were collected and soaked in ethanol solution with a volume fraction of 75%, and stored in a -20°C refrigerator for later use.

[0041] (3) 50K SNP typing of pig whole genome

[0042] The whole genome DNA was extracted from the ear tissue or tail tissue collected from each individual of the 3916 Duroc breeding pigs selected from the above resource groups, and the whole geno...

Embodiment 2

[0059] Example 2 Amplification and sequencing of target DNA sequences

[0060] (1) Primer design

[0061] The DNA sequence of SEQ ID NO: 1 on chromosome 6 of pigs was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html). Primers were designed using primer design software primer premier 6.0. The DNA sequences of the designed primers are shown below:

[0062] P001-F: 5'-ACTCTTGCCTCCGACTTCTC-3',

[0063] P002-R: 5'-AGACCTGGTGACATAGTTGATGA-3';

[0064] (2) PCR amplification

[0065] Add 1 μL of DNA template, 3.4 μL of double-distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R to the 10 μL reaction system. PCR reaction conditions were: 94°C pre-denaturation for 5 min, 35 cycles of 94°C denaturation for 30s, 55°C annealing for 30s, 72°C extension for 45s, and a final extension at 72°C for 5 min.

[0066] (3) DNA sequence determination

[0067] DNA sequence sequencing and identification: carried out in Shenzh...

Embodiment 3

[0070] Example 3 Analysis of T>C effect of molecularly labeled SNP site g.333

[0071] According to Tables 1 to 3, it can be seen that the effect of the SNP locus g.333T>C dominant allele (TT) was significantly increased by 0.62, 0.62, 0.62 and 0.62, respectively, compared with the average value of the CC phenotype, for the lean body percentage, eye muscle area and eye muscle thickness, respectively. 1.28 and 0.76. Therefore, through molecular marker-assisted selection, gradually culling pigs with CC genotype in the population can significantly increase the allele frequency of allele T, and improve the lean meat rate, eye muscle area, and eye muscle thickness of the population. The growth and production performance of pigs are improved, and more high-quality commercial lean pork can be produced to meet people's demand for high-quality pork, thereby driving the growth of pork sales, which will bring huge economic benefits to enterprises.

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Abstract

The invention belongs to the technical field of molecular biotechnology and molecular markers, and in particular relates to a SNP molecular marker on pig chromosome 6 that is related to lean meat percentage, eye muscle area and eye muscle thickness and its application. The SNP site of the molecular marker corresponds to the T>C mutation at position 47357966 on chromosome 6 of the reference sequence of the international pig genome version 11.1. A primer pair for SNP molecular markers related to eye muscle area and eye muscle thickness. By optimizing the dominant allele of the SNP, the present invention can increase the frequency of the dominant allele from generation to generation, improve the lean meat rate of breeding pigs, increase the area and thickness of eye muscles, and synergistically select and breed excellent breeding pigs with the above three traits, thereby accelerating Advances in pig genetic improvement can effectively improve the economics of breeding pigs.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and in particular relates to a SNP molecular marker related to lean meat percentage, eye muscle area and eye muscle thickness on chromosome 6 of pigs and its application. Background technique [0002] In recent years, with the improvement of people's living standards and the demand for pork consumption, lean pigs have occupied a large market share of pork consumption. Therefore, the selection and breeding of high-quality lean pigs has become the focus of breeding work, and the related traits affecting pig growth are important breeding goals. The lean meat percentage of pigs refers to the percentage of lean meat weight in pigs to the whole carcass weight, which is an important indicator to measure the growth performance of pigs. For a long time, benefiting from people's continuous pursuit of lean meat, the lean meat rate has been significantly improved. Eye ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 吴珍芳杨杰庄站伟蔡更元郑恩琴徐铮
Owner SOUTH CHINA AGRI UNIV
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