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Copy number variation molecular marker related to daily gain and market weight day age of pig on pig chromosome 3 and application

A technology of copy number variation and molecular markers, which is applied in the field of molecular biotechnology and molecular markers, can solve problems affecting phenotype, changes, gene dosage effects, gene structures, etc., and achieve the goals of shortening the age, increasing economic benefits, and improving growth performance Effect

Active Publication Date: 2021-03-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the change in copy number, it may lead to a dose effect of the gene or cause a change in the structure of the gene, thereby significantly affecting the corresponding phenotype

Method used

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  • Copy number variation molecular marker related to daily gain and market weight day age of pig on pig chromosome 3 and application
  • Copy number variation molecular marker related to daily gain and market weight day age of pig on pig chromosome 3 and application
  • Copy number variation molecular marker related to daily gain and market weight day age of pig on pig chromosome 3 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Experimental animals

[0046] The group of experimental pigs used in the present invention is 2857 purebred plus line Duroc of Wen's Food Group Co., Ltd. Breeding Pig Branch, which is the core group of Breeding Pig Branch.

[0047] In this experiment, a total of 2857 Canadian Duroc pigs were selected in this resource group. The pigs had free access to food and water, and the entire feeding method and feeding conditions were always consistent, which was a conventional method.

[0048] (2) Sample collection

[0049] The above-mentioned piglet docked tail and ear tissues were collected and soaked in ethanol solution with a volume fraction of 75%, and stored in a -20°C refrigerator for later use.

[0050] (3) 50K SNP typing in the whole pig genome

[0051] The ear tissue or docked tail tissue collected from each of the 2857 Duroc breeding pigs selected from the above-mentioned resource groups, the whole genome DNA was extracted by the standard phenol-chloroform method...

Embodiment 2

[0064] Example 2 Target DNA sequence amplification and sequencing

[0065] (1) Primer design

[0066] Download the specific DNA sequence (SEQ ID NO: 1) located in the 162027bp-2026411bp sequence on the chromosome 3 of the pig as a reference sequence through the NCBI website (http: / / www.ncbi.nlm.nih.gov), and use Primer design software primer premier 6.0 designed qPCR primers (primer pair P1). At the same time, using the porcine GCG gene sequence (NC_010457.5) published by NCBI as a reference sequence, qPCR primers (primer pair P2) were designed using the same method. The DNA sequences of the designed primer pair P1 and P2 are shown below.

[0067] Primer pair P1 is:

[0068] Upstream primer F1: 5'-CTCCCACTCCACCCACCCTAAAG-3',

[0069] Downstream primer R1: 5'-TGCGGACGGAAAGATGAGAACAAG-3';

[0070] Primer pair P2 is:

[0071] Upstream primer F2: 5'-TGCAGAACTACAGGGCTCAGGAC-3',

[0072] Downstream primer R2: 5'-GTGGAACAGGGCAGGTGGAAAG-3';

[0073] (2) qPCR amplification

[...

Embodiment 3

[0085] Example 3 Copy number type qPCR verification and effect analysis of copy number variation molecular markers

[0086] According to Tables 1 and 2, it can be seen that for daily weight gain, the effect of dominant copy number type (normal copy number) in the CNV region is significantly higher than the average value of the copy number gain type phenotype by 8.65; The effect of the copy number type (normal copy number) was significantly shortened by 2.33 compared to the copy number gain type phenotype mean. Therefore, by using molecular marker-assisted selection to gradually eliminate pigs with increased copy number types in the group, the frequency of normal copy number types can be significantly increased, the daily weight gain of the group can be increased, and the age at market weight of the group can be shortened. To short-term high yield, thus bringing huge economic benefits to farming enterprises.

[0087] The present invention detects the copy number variation of t...

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Abstract

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to a copy number variation molecular marker related to daily gain and market weight day age of a pig on a pig chromosome 3 and application. A copy number region of the copy number variation molecular marker related to the daily gain and the market weight day age of the pig on thepig chromosome 3 corresponds to a fragment, located in a 162027bp-2026411bp interval, on the chromosome 3 of a reference sequence of an international pig genome version 11.1, and copy number variation is prone to occurring in the interval. According to the copy number variation molecular marker related to the daily gain and the market weight day age of the pig on the pig chromosome 3 and the application, by optimizing the dominant copy number type of the copy number variation molecular marker, the frequency of the dominant copy number type can be increased generation by generation, thus the daily gain is increased, and the market weight day age is shortened; and excellent breeding pigs with the two characters are bred cooperatively, and the economic benefits of breeding of the breeding pigs can be effectively increased while the genetic breeding progress of the pigs can be accelerated.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and specifically relates to a molecular marker of copy number variation on pig No. 3 chromosome that is related to pig daily gain and weight-on-market age and its application. Background technique [0002] Pork is an important source of animal protein in my country, and its consumption accounts for more than 60% of my country's meat consumption market. Therefore, some important economic traits of pigs, such as growth, reproduction and meat quality traits, have been studied and improved by breeders in my country for a long time. In recent years, with the continuous growth of people's pork consumption level and the transformation of consumption concepts, lean pork has gradually dominated the pork consumption market in my country. Therefore, the breeding of high-quality lean pigs has become the focus of breeding work, and the relevant traits that affect pig gro...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/156C12Q2600/124C12Q2531/113C12Q2545/101C12Q2537/16
Inventor 蔡更元吴珍芳杨杰陈悦郑恩琴庄站伟丁荣荣邱益彬吴杰杨化强洪林君黄思秀
Owner SOUTH CHINA AGRI UNIV
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