Copy number variation molecular marker related to daily gain and market weight day age of pig on pig chromosome 3 and application

A technology of copy number variation and molecular markers, which is applied in the field of molecular biotechnology and molecular markers, can solve problems affecting phenotype, changes, gene dosage effects, gene structures, etc., and achieve the goals of shortening the age, increasing economic benefits, and improving growth performance Effect

Active Publication Date: 2021-03-09
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the change in copy number, it may lead to a dose effect of the gene or cause a chang

Method used

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  • Copy number variation molecular marker related to daily gain and market weight day age of pig on pig chromosome 3 and application
  • Copy number variation molecular marker related to daily gain and market weight day age of pig on pig chromosome 3 and application
  • Copy number variation molecular marker related to daily gain and market weight day age of pig on pig chromosome 3 and application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1

[0045] (1) Experimental animals

[0046] The experimental pig population used in the present invention is a purebred plus of the Wildlin Food Group Co., Ltd. Pig Branch, which is 2,857, for the core group of pig branch.

[0047] This experiment collects 2857 heads in the resource group to add Dolock breeding pigs, and the pigs are free to feed, drinking water, the whole feeding method, and the feeding conditions, etc., which are conventional methods.

[0048] (2) Sample collection

[0049] Collecting the above-mentioned pigtail and ear tissue soaking in a solution of a volume fraction of 75%, placed in the -20 ° C refrigerator preservation spare.

[0050] (3) Pig whole genome 50K SNP sentence

[0051] From the 2,857-headed Dolock species selected from the above resource groups, the ear tissue or broken tissue collected by the standard phenol-chloroform is extracted with standard phenol-chloroform, and each part is accurately determined by the NanoDrop2000 / 2000...

Example Embodiment

[0064] Example 2 Purpose DNA sequence amplification and sequencing

[0065] (1) primer design

[0066] Download the 162027 bp-2026411 bp sequence (SEQ ID NO: 1) in the 162027 bp-2026411 bp sequence (SEQ ID NO: 1) in the 162027 bp-2026411 bp sequence (SEQ ID NO: 1) in the 162027 bp-2026411 bp sequence, and utilized Primer Design Software Primer PREMER 6.0 Design QPCR primer (primer pair P1). At the same time, the Pig GCG gene sequence (NC_010457.5) published by NCBI is used as a reference sequence, and the same method is used to design the same method (primer pair P2). The designed primer for the DNA sequences of P1 and P2 are as follows.

[0067] Primers pair P1 is:

[0068] Upstream primers F1: 5'-ctcccactcacccccccccctaaag-3 ',

[0069] Downstream primers R1: 5'-tgcggacgaaagatgagaacaag-3 ';

[0070] Primers pair P2 is:

[0071] Upstream primer F2: 5'-tgcagaactacagggctcaggac-3 ',

[0072] Downstream primers R2: 5'-gtggaacaggcaggtgggaaaag-3 ';

[0073] (2) QPCR amplification

[007...

Example Embodiment

[0085] Example 3 Copy number type QPCR verification and effect analysis of copy number variant molecular markers

[0086] According to Table 1 to 2, the effect of the advantageous copy number (normal copy number) in the CNV region is significantly increased by 8.65, which increases the average value of the copy number increased by 8.65; for the listing weight, the advantage in the CNV area The effect of the copy number (normal copy number) is significantly shortened than the copy number increasing phenotype. Therefore, by molecular marking assistance, gradually eliminate the number of copies of the copy number in the population to increase the number of copies of the number of copies, and improve the daily gain of the group, shorten the group's listing weight, effectively By short-term high yield, thereby brings huge economic benefits to farming companies.

[0087] The present invention performs detection of copy number variation of the SEQ ID NO: 1 sequence, preliminary perceptio...

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Abstract

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to a copy number variation molecular marker related to daily gain and market weight day age of a pig on a pig chromosome 3 and application. A copy number region of the copy number variation molecular marker related to the daily gain and the market weight day age of the pig on thepig chromosome 3 corresponds to a fragment, located in a 162027bp-2026411bp interval, on the chromosome 3 of a reference sequence of an international pig genome version 11.1, and copy number variation is prone to occurring in the interval. According to the copy number variation molecular marker related to the daily gain and the market weight day age of the pig on the pig chromosome 3 and the application, by optimizing the dominant copy number type of the copy number variation molecular marker, the frequency of the dominant copy number type can be increased generation by generation, thus the daily gain is increased, and the market weight day age is shortened; and excellent breeding pigs with the two characters are bred cooperatively, and the economic benefits of breeding of the breeding pigs can be effectively increased while the genetic breeding progress of the pigs can be accelerated.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and specifically relates to a molecular marker of copy number variation on pig No. 3 chromosome that is related to pig daily gain and weight-on-market age and its application. Background technique [0002] Pork is an important source of animal protein in my country, and its consumption accounts for more than 60% of my country's meat consumption market. Therefore, some important economic traits of pigs, such as growth, reproduction and meat quality traits, have been studied and improved by breeders in my country for a long time. In recent years, with the continuous growth of people's pork consumption level and the transformation of consumption concepts, lean pork has gradually dominated the pork consumption market in my country. Therefore, the breeding of high-quality lean pigs has become the focus of breeding work, and the relevant traits that affect pig gro...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/156C12Q2600/124C12Q2531/113C12Q2545/101C12Q2537/16
Inventor 蔡更元吴珍芳杨杰陈悦郑恩琴庄站伟丁荣荣邱益彬吴杰杨化强洪林君黄思秀
Owner SOUTH CHINA AGRI UNIV
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