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Molecular lock construction method based on Nickering enzyme

A construction method and technology of DNA molecules, applied in the field of biological computing, can solve the problem of reducing the security of molecular locks

Pending Publication Date: 2021-01-29
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the security of the molecular lock is greatly reduced

Method used

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  • Molecular lock construction method based on Nickering enzyme
  • Molecular lock construction method based on Nickering enzyme
  • Molecular lock construction method based on Nickering enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The construction of embodiment 1 logic calculation module

[0025] according to figure 1 The construction method of the logical calculation module in image 3 The result analysis of the logic calculation module is given. We can see that when an input is present, a strong output signal is produced. Conversely, when there is no input signal, the computing module does not generate an output signal. It shows that the logical computing module we constructed can respond correctly to the input.

Embodiment 2

[0026] Embodiment 2 suppresses the construction of module

[0027] according to figure 2 In the constructor of the suppression module, Figure 4 The analysis of the results of the suppression module is given. We can see that when input A is present, the block produces a distinct output signal. Conversely, when input signal B is present and both A and B are present, no significant output signal is produced. Comparing the case with no input, the results are in stark contrast. It shows that the suppression module can give correct output results for four kinds of inputs.

Embodiment 3

[0028] The construction of embodiment 3DNA molecular lock

[0029] Finally we give the result of DNA molecular lock, as Figure 5 shown. We set the correct password as A-B-C, we can see that only when A-B-C is entered, the molecular lock will give a response. In contrast, the other five outcomes had no apparent output response. Compared with the situation without input, it fully demonstrates that the molecular lock device we constructed can respond correctly to the correct input.

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Abstract

The invention discloses a molecular lock construction method based on Nickering enzyme. The molecular lock construction method comprises the following steps: constructing a logic calculation module through a DNA strand displacement technology according to a cutting characteristic principle of the Nickering enzyme; designing the position of a Nickering enzyme recognition domain so as to form an inhibition effect of two Nickering enzymes and construct an inhibition module; and constructing a DNA molecular lock according to the cutting characteristics of the Nickering enzyme and the inhibition module. The method comprises the following steps: firstly, constructing a logic calculation module through the cutting characteristics of Nickering enzyme; then constructing an inhibition module by utilizing the interaction of the plurality of Nickering enzymes, and finally constructing a DNA molecular lock with a self-destruction protection mechanism based on the Nickering enzymes on the basis of the constructed logic calculation module.

Description

technical field [0001] The invention relates to the field of biological computing, in particular to a Nicking enzyme-based molecular lock construction method. Background technique [0002] In recent years, DNA nanotechnology has achieved rapid development, especially the combination of DNA molecules with ribozymes, restriction endonucleases, metal ions and other materials, making DNA molecules widely used in the construction of various nano-devices. Among them, Nicking enzyme, as a restriction endonuclease, can specifically recognize DNA double strands and cut corresponding positions, thereby promoting or inhibiting the reaction. Taking advantage of this property, Nicking enzyme has become a powerful tool for building DNA molecular devices. As people pay more and more attention to information security, how to realize information protection at the molecular level has become the current focus. Molecular lock, as an intelligent molecular sensor, has shown great potential for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B45/00G16B40/10G16B15/00C12N9/22
CPCG16B40/10G16B15/00G16B45/00C12N9/22
Inventor 张强张晓康王宾魏小鹏
Owner DALIAN UNIV