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Environment-friendly enzyme capable of resisting pig farm liquid dung alkaline environment and preparation method

An environmentally friendly enzyme, alkaline environment technology, applied in microorganism-based methods, biochemical equipment and methods, separation methods, etc., can solve the problem of no relevant reports, and achieve the effect of improving the breeding environment and achieving excellent results.

Pending Publication Date: 2021-02-05
ANHUI TECH BANK BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the use of Bacillus licheniformis as an environmental protection agent, and there are basically no relevant reports in China

Method used

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  • Environment-friendly enzyme capable of resisting pig farm liquid dung alkaline environment and preparation method
  • Environment-friendly enzyme capable of resisting pig farm liquid dung alkaline environment and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0022]Purpose and significance: To investigate the effect of the environmentally friendly enzyme of the invention on the ammonia concentration in a pig house.

[0023]Test site: Han Shiwei Bengbu Weizhuang Pig Farm.

[0024]There are 2 pens in the pig farm. Pens 1-40 are in pen 1, which is the experimental group; pens 41-80 are in pen 2 and are the control group; 40kg of environmentally friendly enzymes are diluted 10 times and sprayed evenly on the floor of the experimental group ; The control group is not treated; during the experiment, the doors, windows and fans of the two stables are closed to achieve the purpose of separation; the ammonia measurement point is set at the center of the corresponding stable, 1.2m from the ground, and the Greek Mamma ammonia gas detector for testing.

[0025]The experimental results are as follows:

[0026]

[0027]It can be seen from the above table: Compared with the control group, the ammonia concentration of the experimental group was significantly reduced, ...

Embodiment example 2

[0029]Purpose and significance: To investigate the influence of the environmental protection enzyme of the present invention on the ammonia concentration of the environmental protection sewage of the pig farm.

[0030]Test site: Hanshiwei Huanghuatang Pig Farm Environmental Protection Zone.

[0031]The control group was the average of 10 tests in the normal process. The experimental group added the environmentally friendly enzyme lactic acid bacteria to the anaerobic reactor at an added amount of 0.5%. The sewage was fermented in the anaerobic reactor for a week, and the ammonia nitrogen, COD content: According to the normal process, when the sewage enters the two-stage A / O tank, add Bacillus at 0.5%, and aerate for 6-7 days to detect ammonia nitrogen and COD.

[0032]The experimental results are as follows:

[0033]

[0034]It can be seen from the above table: Compared with the control group, the concentration of ammonia nitrogen and COD in the experimental group was significantly lower, showing ...

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Abstract

The invention discloses environment-friendly enzyme capable of resisting pig farm liquid dung alkaline environment and a preparation method. The preparation method comprises the following steps: inoculating a beef extract peptone culture medium with a strain of NA , performing shaking culture at 37 DEG C for 12-18 hours to form a seed solution, performing large-tank fermentation for 24-48 hours, and adding fermented bacillus subtilis and lactic acid bacteria before sub-packaging, thereby obtaining the environment-friendly enzyme. The NA obtained through on-site enrichment and screening can grow and proliferate by taking pig farm liquid dung as a main nutrient, and ammonia nitrogen and total nitrogen can be obviously reduced. The NA is used as main materials, and the bacillus subtilis, saccharomycetes, lactic acid bacteria and the like are used as auxiliary materials to produce the environment-friendly enzyme. The product can be directly diluted by 10-30 times and then sprayed into a pig house, the effects of deodorization and ammonia gas reduction are achieved, the breeding environment is improved, and the effect of the enzyme is superior to that of similar products on the market.

Description

Technical field[0001]The invention relates to the technical field of environmental protection enzymes, in particular to an environmental protection enzyme resistant to the alkaline environment of pig farm manure water and a preparation method.Background technique[0002]Ammonia is a strongly irritating gas. According to research reports, the average ammonia emission per fattening pig is 107.18-424.42mg / h, and a pig farm that produces 10,000 pigs a year has an average daily nitrogen emission of 105kg, of which a large part is ammonia (NH3) Is released into the atmosphere. Excessive concentration of ammonia in the pig house will not only affect the growth and development of pigs, but also reduce the body’s resistance, which will induce a variety of diseases and cause serious economic losses to pig production. At the same time, it will affect the breeder and the surrounding environment of the pig farm. It also caused harm.[0003]Pigs living in a low-concentration ammonia environment for a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16B01D53/78B01D53/58B01D53/54A01K1/00C12R1/01
CPCC12N1/20C12N1/16B01D53/78B01D53/58B01D53/54A01K1/0047B01D2258/0266B01D2257/406
Inventor 张邦辉胡传炯
Owner ANHUI TECH BANK BIO TECH
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