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Liquid fermentation method for increasing yield of phellinus igniarius mycelia

A technology of Phellinus linteus mycelium and liquid fermentation, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve problems such as general low yield, and achieve the effect of increasing yield

Pending Publication Date: 2021-02-05
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the liquid culture of Phellinus Phellinus, the current technology shows that the yield is generally low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A liquid fermentation method for improving the output of Phellinus phellidis mycelia, comprising:

[0025] S1: The liquid medium includes the following components by weight: 20g of maltose, 20g of yeast powder, 10g of yam, 10g of soybean powder, 10g of potato powder, 0.5g of triammonium citrate, and 0.002g of vitamin B, configured as a 200ml liquid Culture medium;

[0026] S2, Phellinus bacterium is inoculated in the liquid culture medium, carries out activation culture, then receives in the seed culture medium by the 12% inoculum amount of volume percentage, carries out seed culture, obtains seed liquid;

[0027] S3. Inoculate the seed solution prepared by S2 into a liquid culture medium with an inoculum volume of 10-15% by volume. Under the condition of a temperature of 30°C, the liquid fermentation culture is carried out for 2 days, and a certain amount of Vegetable oil and magnesium acetate tetrahydrate, and then continue to cultivate for 3 days at a temperature of...

Embodiment 2

[0031] The difference from Example 1 is that the liquid medium includes the following components by weight: 30 g of maltose, 30 g of yeast powder, 20 g of Chinese yam, 20 g of soybean powder, 20 g of potato powder, 1 g of triammonium citrate, and 0.005 g of vitamin B, It is configured as 300-400ml liquid medium.

Embodiment 3

[0033] The difference from Example 1 is that the liquid medium includes the following components by weight: 25g of maltose, 25g of yeast powder, 15g of Chinese yam, 15g of soybean powder, 15g of potato powder, 0.8g of triammonium citrate, and 0.004g of vitamin B , configured as 200-300ml liquid medium.

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PUM

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Abstract

The invention discloses a liquid fermentation method for increasing the yield of phellinus igniarius mycelia. The liquid fermentation method comprises the following steps of S1, preparing a liquid culture medium for phellinus igniarius culture; S2, inoculating phellinus igniarius strain into the liquid culture medium, performing activation culture, inoculating into a seed culture medium accordingto the inoculation amount of 10-15% by volume, and performing seed culture to obtain a seed solution; and S3, inoculating the seed solution prepared in the step S2 into a liquid culture medium according to the inoculation amount of 10-15% by volume, performing liquid fermentation culture for 1-3 days at 28-35 DEG C, adding a certain amount of vegetable oil and magnesium acetate tetrahydrate in anyday in the culture process, continuously culturing for 2-3 days at 23-28 DEG C to obtain a mixed solution of the phellinus igniarius mycelium and fermentation liquor. The invention aims to provide the liquid fermentation method for improving the yield of the phellinus igniarius mycelium, and the yield is effectively improved.

Description

technical field [0001] The invention relates to a liquid fermentation method for increasing the yield of Phellinus mycelium. Background technique [0002] Phellinus is a perennial medicinal fungus, and it is one of the most promising rare and endangered traditional Chinese medicines in China. It is mainly distributed in the "Ziwuling" area at the junction of Qinling, Shaanxi and Gansu. It grows on dead standing trees and trunks of broad-leaved trees such as poplar, mulberry, willow, birch, zelkova, and peach. "Dictionary of Chinese Medicine" records that it can cure gynecological diseases such as metrorrhagia, bloody stranguria, leukorrhea, and amenorrhea. Modern studies have found that Phellinus has obvious anti-hepatic fibrosis effects, and is currently internationally recognized as the highest effective rate in the field of biological cancer treatment of higher fungi. [0003] At present, most of Phellinus chinensis products depend on the fruiting bodies of Phellinus c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/14C12R1/645
CPCC12N1/38C12N1/14
Inventor 雷萍吴亚召张文隽马婧嘉黄文静渠敬峰钱磊
Owner MICROBIOLOGY INST OF SHAANXI
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