Two-time shifting cell migration assay method

An assay method and cell migration technology, applied in the field of cell biology, can solve problems such as high cost, unclean erasing, and time-consuming, and achieve the effects of avoiding errors, shortening cell migration distance, and eliminating manual delimitation.

Active Publication Date: 2021-02-09
PUTIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the need to manually erase the cells retained in the cup, it takes a long time and cannot avoid the problem of unclean erasure. At the same time, the price of the porous polycarbonate membrane cup is relatively expensive, and the number of porous p

Method used

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  • Two-time shifting cell migration assay method
  • Two-time shifting cell migration assay method
  • Two-time shifting cell migration assay method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030]Example 1:

[0031]referenceFigure 1-2 , The method for measuring the migration of two translocation cells, specifically includes the following steps:

[0032]Step 1. Place the nipple slides in the culture holes of the first 96-well cell culture plate in turn, and then insert 100μL of cells to be tested into each hole (the culture hole of the culture plate) for cell culture, and form 2-4x10 the next day4The density of cells / well;

[0033]Step 2. After the cell culture is over (80-100% full), take out the nipple slides one by one, and use sterilized tweezers to pick up the nipple slides and place them in the culture wells of the second 96-well cell culture plate. Transplant once, then add cell culture medium and test substances (such as organic reagents, inorganic reagents, peptides, biologically active substance inhibitors, stimulants, etc.), and culture for 12-24 hours. At this time, the cells migrate and the migrated cells pass through the nipple The edge of the glass slide and the t...

Example Embodiment

[0038]Example 2:

[0039]referenceFigure 3-4, The method for measuring the migration of two translocation cells, specifically includes the following steps:

[0040]Step 1. Place the nipple slide in the culture hole of the first 48-well cell culture dish, and then insert 200μL of the cells to be tested into each hole (culture hole of the culture dish) for cell culture, and form 6-8x10 the next day4The density of cells / well;

[0041]Step 2. After the cell culture is over, take out the nipple slides one by one, place 48 nipple slides in the culture holes of the second 48-well cell culture plate in turn, complete the first shift, and then add the cell culture medium and the test substance (Such as organic reagents, inorganic reagents, peptides, biologically active substance inhibitors, stimulants, etc.), culture for 12-24h, at this time the cells will migrate;

[0042]Step 3. Take the nipple slides out of the second 48-well cell culture plate and complete the second shift. The cells left in the sec...

Example Embodiment

[0046]Example 3:

[0047]The difference between Embodiment 3 and Embodiment 1 is:

[0048]The first cell culture tray and the second cell culture tray are both 24-well cell culture trays;

[0049]The material of the nipple glass slide is polyethylene resin;

[0050]The through holes are strip-shaped, the nipple glass slide has a thickness of 0.5 mm, the nipple glass slide is a cuboid, and the length of the nipple glass slide is smaller than the diameter of the culture hole of the 24-well cell culture plate.

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Abstract

The invention belongs to the technical field of cell biology, and discloses a two-time shifting cell migration assay method. The method comprises the following steps: placing a nipple slide in a culture hole of a first cell culture disc, and inoculating a cell to be determined; taking out the nipple slide after culturing, and placing the nipple slide in a culture hole of a second cell culture disc; adding a cell culture solution and an experimental substance; and taking out the nipple slide, and performing quantification through an XTT colorimetric method or an MTT colorimetric method. The nipple slide comprises a slide body and a mastoid arranged at the center of the slide body, and a plurality of through holes are formed in the slide body; and the nipple slide is made of polymer resin. The two-time shifting cell migration assay method is slightly influenced by manual operation, the cell migration distance is short, the time is short, the method is not easily influenced by cell proliferation, used equipment is generally arranged in laboratories, the required cost is low, and common laboratories can autonomously design and finish the required cell migration assay according to the provided two-time shifting cell migration assay method.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to a method for measuring the migration of twice-shifted cells. Background technique [0002] Cell migration assays are one of the fundamental methods for probing the behavioral capabilities of cells in cell biology research. It is widely used in cell biology research. Through the study of cell migration ability, we can understand the mechanism of physiological and pathological processes, such as angiogenesis, embryonic development, immune cell function, tumor cell migration, wound healing, etc. [0003] There are two methods commonly used to measure cell migration. The first is the wound healing experiment. In this method, the cells planted on the glass slide are artificially scraped off a 1-2mm wide blank area, and the boundary is marked with a marker pen, and the slide is placed in the culture medium of the test condition. Cultivate for a certain period of tim...

Claims

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Application Information

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IPC IPC(8): C12Q1/06G01N21/31
CPCG01N33/5029G01N21/31
Inventor 朱棠
Owner PUTIAN UNIV
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