Spermatogonial stem cell cryopreservation system and spermatogonial stem cell cryopreservation method

Abstract

The invention discloses a spermatogonial stem cell cryopreservation system and a spermatogonial stem cell cryopreservation method. The spermatogonial stem cell cryopreservation system comprises a base, a bearing column rotationally connected with the base, and storage tubes distributed in the bearing column in a circumferential array mode. Cavities distributed in a circumferential array mode are formed in the side, away from the base, of the bearing column, long-strip-shaped openings are formed in the sides, located on the surface of the bearing column, of the cavities, the length of the cavities is equal to that of the storage tubes, and the storage tubes are movably connected with the cavities. In using process of the system, sperm cells can be directly stored in storage chambers in thestorage tubes, each storage tube is provided with a plurality of storage chambers, so that one storage tube can store multiple human sperm cells, the storage quantity of the system is greatly increased, meanwhile, due to the design of the cylinder shape, the system can store a large number of sperm cells, the overall structure is compact, the space of a liquid nitrogen container can be fully utilized, and the utilization efficiency of the liquid nitrogen tank is greatly improved.

Description

technical field [0001] The invention relates to the technical field of spermatogonial stem cell storage, in particular to a spermatogonial stem cell cryopreservation system and a preservation method thereof. Background technique [0002] Spermatogonial stem cells cannot fertilize eggs, but they produce cells that can develop into sperm. At puberty, human testicles are stimulated by pituitary gonadotropin, spermatogonial stem cells start to activate, spermatogonial cells continue to proliferate and develop, and evolve into sperm. Spermatogenesis is firstly the regeneration of undifferentiated spermatogonia, and the division and proliferation of spermatogonia. After several mitosis of spermatogonia, some of them grow into primary spermatocytes, and the other part remains as stem cells. The primary spermatocytes divide for the first time to form two secondary spermatocytes, and undergo the second mature division to form two spermatids. [0003] Spermatogonia Stem Cells (SSCs...

AI Technical Summary

Problems solved by technology

[0005] At present, the existing spermatogonial stem cell storage method is to put the cryopreservation bag into a cylindrical container, and then put it into a liquid nitrogen tank for storage. Since the shape of the cryopreservation bag and the cylindrical container do not match, the During cryopreservation, it will cause deformation of the cryopreservation bag, causing extrusion damage to the spermatogonial stem cells, and the amount that can be frozen is extremely limited, which increases the cost of purchasing a liquid nitrogen tank. If you purchase a cell cryopreservation solution separately Nitrogen tank, on the basis that the liquid nitrogen tank does not have enough efficiency, the liquid nitrogen tank for cell freezing every day will also cause a large amount of liquid nitrogen to volatilize, which also greatly increases the daily cost of liquid nitrogen, causing a lot of waste

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