A method for constructing cyp17a1 Cre animal model based on CRISPR-Cas9

An animal model, the technology of p2a-cre, applied in the field of biomedicine, can solve the problems of random insertion position, low construction efficiency, unstable Cre enzyme expression, etc., achieve good stability and specificity, good economic benefits, and overcome vector insertion. position random effect
CN112410337BActive Publication Date: 2022-04-12SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2022-04-12

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Abstract

The present invention provides a method for constructing a Cyp17a1 Cre animal model based on CRISPR-Cas9. The Cyp17a1 gene is transformed to obtain a Cre enzyme expression model mouse. In the existing model animal construction method, there are random insertion positions, low construction efficiency, and Cre enzyme expression. Insufficient expression such as instability. In view of the above-mentioned technical defects, the present invention provides a method for constructing a Cyp17a1 Cre animal model, using CRISPR-Cas9 technology, according to the principle of homologous recombination, inserting the Cre enzyme expression sequence at a fixed point before the stop codon TAG of the Cyp17a1 gene, accurately and efficiently A new Cyp17a1 Cre tool mouse was constructed and the stability and specificity of its Cre enzyme expression were verified.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for constructing a Cyp17a1 Cre animal model based on CRISPR-Cas9. Background technique

[0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] The ovary is an important reproductive organ in female animals, and its main functions include producing eggs and secreting sex hormones. The sex hormones secreted by the ovary are mainly estrogen and progesterone, in addition to a certain amount of androgen. They all belong to steroid hormones and have a common synthetic pathway: pregnenolone derived from cholesterol is converted into androgen (testosterone) by aromatase in theca cells, a...

Claims

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