A disease-resistant locus qsrv6.1, molecular markers and applications of black-streaked dwarf disease in southern rice

A technique for black-streaked dwarfism and molecular markers, applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc.

Active Publication Date: 2021-09-10
RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no effective disease-resistant locus for the creation of new rice varieties. Therefore, mining SRBSDV disease-resistant loci, verifying their effectiveness, and applying them to germplasm creation is an urgent problem to be solved.

Method used

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  • A disease-resistant locus qsrv6.1, molecular markers and applications of black-streaked dwarf disease in southern rice
  • A disease-resistant locus qsrv6.1, molecular markers and applications of black-streaked dwarf disease in southern rice
  • A disease-resistant locus qsrv6.1, molecular markers and applications of black-streaked dwarf disease in southern rice

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Experimental program
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Effect test

Embodiment 1

[0026] 1. Mining of resistance loci to southern rice black-streaked dwarf disease (SRBSDV)

[0027] The identification of SRBSDV resistance adopts the field natural induction identification method. 500 rice resources were planted in the SRBSDV disease nursery in Xiangli Town, Xing'an County, Guangxi. No pesticides were sprayed during the seedling field stage, and seedlings were transplanted per plant. Each variety was planted in 4 rows*10 plants with a row spacing of 16cm*18cm. The disease-resistant variety was W44, and the control susceptible variety was TN1. All varieties were set 3 repetitions, 3 repetitions were randomly arranged in 6 fields according to the numbering order of the tested varieties, and 5 TN1 plants were planted in protection rows and induced rows around the fields, with a plant spacing of 10cm, and only one insecticide was sprayed during the entire growth period (insecticide Single) Control rice leaf roller. About 30 days after transplanting, the phenoty...

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Abstract

The invention discloses a rice black-streaked dwarf resistance locus qSRV6.1 and its application. qSRV6.1 is located at the physical location of 0.91‑1.18 Mb in the rice Nipponbare reference genome (IRGSP‑1.0). Sequencing analysis found that compared with the susceptible variety Nipponbare, the resistant variety W44 inserted 25 nucleotides after 926852bp of the Nipponbare genome, 12 nucleotides were inserted after 1171606bp of the Nipponbare genome, and 12 nucleotides were inserted after 1171647bp of the Nipponbare genome. The insertion inserted 9 nucleotides. The molecular markers qSRV6.1-1 and qSRV6.1-2 closely linked to the disease-resistant locus qSRV6.1 can be effectively used to distinguish disease-resistant varieties from susceptible varieties, and can be used to assist molecular markers in the process of cross-breeding choose. The invention proves that the disease resistance locus qSRV6.1 can significantly improve the SRBSDV resistance of rice through experiments, and has important value in the application of rice disease resistance.

Description

Technical field: [0001] The invention belongs to the field of rice disease resistance, and in particular relates to a disease-resistant locus, a molecular marker and an application of southern rice black-streaked dwarf disease. Background technique: [0002] Since Southern rice blackstreaked dwarfvirus (SRBSDV) was first discovered in Yangxi County, Guangdong Province in 2001, the occurrence area has been expanding year by year. In 2009, the disease commonly occurred in 9 provinces (autonomous regions) in southern my country and 19 provinces in northern Vietnam. After the rice was infected, the yield loss exceeded 50%, and even the grains failed. The disease is spread over long distances by the migratory pest white-backed planthopper and poses a major threat to rice production in my country. In view of the harmfulness caused by SRBSDV, it is an urgent task for plant protection departments at all levels to formulate effective prevention and control measures for the disease. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵均良刘志霞秦碧霞杨武陈洛周炼刘斌白嵩
Owner RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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