Method of assessing circulating tumor cells and use thereof

A tumor cell and information evaluation technology, which is applied in the field of circulating tumor cell information evaluation, can solve the problem of missing circulating tumor cells and achieve precise results for clinical research purposes

Pending Publication Date: 2021-03-05
CHANG GUNG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is known that the particle size of Circulating Tumor Cells (CTCs) varies, so there is a risk of missing Circulating Tumor Cells (CTCs) with a particle size similar to that of white blood cells.

Method used

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  • Method of assessing circulating tumor cells and use thereof
  • Method of assessing circulating tumor cells and use thereof
  • Method of assessing circulating tumor cells and use thereof

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Embodiment Construction

[0033] In order to understand the technical features and practical effects of the present invention, and to implement it according to the contents of the specification, the preferred embodiment shown in the drawings will be described in detail as follows:

[0034] Please refer to figure 1 , figure 1 It is a flow chart of the method for evaluating circulating tumor cell information according to the embodiment of the present invention. Such as figure 1 As shown, this embodiment mainly includes steps (a) to (e). Wherein, in step (a), a biological sample is provided first, and the biological sample includes a plurality of non-target cells and a plurality of target cells. The next step (b) is to screen out the plurality of non-target cells to keep the plurality of target cells alive.

[0035] And step (c) is followed by performing an immunofluorescence staining on the biological sample to distinguish the plurality of circulating tumor cells among the plurality of target cells. ...

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Abstract

Methods of assessing circulating tumor cells (CTCs) and methods of using the data obtained from assessing CTCs are provided. The methods employ negative selection and 3D cell culture techniques to isolate and culture CTCs. Those CTCs then can be used in different bioassays or evaluations, such as to determine tumor morphology, monitor tumor status, predict prognosis, provide treatment suggestion,and assess treatment effectiveness.

Description

technical field [0001] The present invention relates to a circulating tumor cell information evaluation method and its application, especially to a circulating tumor cell information evaluation method that can evaluate the future development possibility and status of tumors through special nucleated cell type screening and quantitative analysis techniques and its use. Background technique [0002] Circulating Tumor Cells (CTCs) are one of the categories of liquid slices. It is often used to evaluate the number in blood samples, and is used in clinical diagnosis, prognosis, evaluation of treatment effectiveness or disease monitoring and other purposes. However, technological breakthroughs are urgently needed to overcome some inherent characteristics of circulating tumor cells (CTCs), such as the extremely rare number and high heterogeneity, which cause difficulties in application. [0003] Circulating Tumor Cells (CTCs) as samples can be used in basic research, cell expansi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/30
CPCG01N21/6428G01N1/30G01N2021/6439C12N5/0693C12N2513/00G01N2800/56G01N2800/52G01N33/5091G01N33/574G01N33/57492G01N33/582G01N2333/4742C12Q1/6886C12Q2600/158G01N33/5094C12Q1/6806
Inventor 吴旻宪廖家蓉
Owner CHANG GUNG UNIVERSITY
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