Preparation method of artificial antibody for detecting heavy metal ions

Abstract

The invention relates to a preparation method of an artificial antibody for detecting heavy metal ions. The surface of a nano SiO2 sphere in the synthesized artificial antibody is coated with an acrylamide and ethylene glycol dimethacrylate polymer shell layer to form a core-shell structure, and the method is characterized in that an imprinting shell layer recognition site is modified with a fluorescent probe; recognition sites in the heavy metal ion artificial antibody shell are eluted to form a cavity structure matched with an imprinted heavy metal ion space structure, the heavy metal ions reenter the recognition sites, and electrons on a recognition site fluorescent probe are captured by utilizing a fluorescence resonance energy transfer mechanism; and high-selectivity recognition and high-sensitivity detection on heavy metal ions are realized. According to the invention, the nano synthesis technology, the molecular imprinting technology and the material surface post-functional modification technology are combined, the key scientific problem that a traditional molecular imprinting material has no signal output is solved, and the method is simple, good in selectivity, high in sensitivity and broad in spectrum for the heavy metal ions.

Description

technical field [0001] The invention relates to the field of material science, in particular to a fluorescent probe artificial antibody SiO for detecting heavy metal ions 2 @MIPs preparation method. Background technique [0002] In recent decades of rapid economic development in my country, industrial "three wastes" and motor vehicle exhaust emissions, sewage irrigation, use of pesticides, herbicides, fertilizers, etc., and the development of mining have led to a large amount of heavy metals entering the soil and water. According to statistics, about 20% of the land in my country has been seriously polluted by heavy metals, and its total area is about 11 million km 2 ; The total amount of heavy metal pollutants in rivers such as the Yangtze River, Pearl River, and Yellow River is about 34,000 tons. Lead, copper, mercury, and cadmium in seawater samples from coastal waters all over the country exceeded the standard. Among them, the rate of copper exceeding the standard was ...

AI Technical Summary

Problems solved by technology

[0008] To sum up, the existing imprinted polymers still have some difficulties to be overcome in practical applications such as molecular recognition or signal output, which can be summarized as follows: (1) Due to the small number of effective sites, Low affinity for target molecules; (2) Molecularly imprinted polymers are usually irregularly shaped materials, which have poor compatibility with sensing devices

(3) The target analyte cannot be exported as a sensitive signal when it enters the recognition site

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