A kind of lactoferrin nanoparticle carrying rhein and its preparation method and use

A technology of lactoferrin and nanoparticles, which is applied in the field of lactoferrin nanoparticles loaded with rhein and its preparation, can solve the problems of low bioavailability, poor colon targeting ability, and low water solubility, and achieve rich sources, Improvement of colitis, the effect of simple preparation method

Active Publication Date: 2022-07-12
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Description
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Problems solved by technology

[0004] However, there are some problems when rhein (RH) is used, such as: low water solubility, low bioavailability, poor colon targeting ability

Method used

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  • A kind of lactoferrin nanoparticle carrying rhein and its preparation method and use
  • A kind of lactoferrin nanoparticle carrying rhein and its preparation method and use
  • A kind of lactoferrin nanoparticle carrying rhein and its preparation method and use

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Embodiment 1

[0070] Embodiment 1, the preparation of lactoferrin rhein nanoparticles of the present invention

[0071]Lactoferrin-rhein nanoparticles (rhein-loaded lactoferrin nanoparticles) were prepared by dialysis technology. Lactoferrin (LF) 50 mg was dissolved in 20 mL of deionized water to prepare an LF solution. Meanwhile, 5 mg of rhein (RH) was completely dissolved in 1 mL of DMSO to obtain an RH solution. The above RH solution was added dropwise to the LF solution under constant magnetic stirring. The mixture was then placed in an ice bath and sonicated using a probe sonicator (Sonicator XL, Misonix, Melville, NY, USA) at 50% amplitude power for 5 minutes to form an emulsion. Then, the emulsion was dialyzed against deionized water (molecular weight cut-off of 1000 Da) for 6 h to remove DMSO. Subsequently, it was filtered with a 0.45 μm filter to remove dust and free drug, and RH-NPs were obtained after filtration.

[0072] After that, 20 mg of hyaluronic acid (HA) was added to...

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Abstract

The invention provides a rhein acid-loaded lactoferrin nanoparticle, a preparation method and application thereof, and belongs to the technical field of biomedical materials. Specifically, a drug-loaded lactoferrin nanoparticle is provided, which is a nanoparticle with a three-layer structure; the three-layer structure is a pectin layer, a hyaluronic acid layer, and a drug-loaded lactoferrin layer from outside to inside. The drug is preferably rhein. The drug-loaded dual-targeted drug delivery nanoparticle of the present invention has good biocompatibility, good stability, can be administered orally, and has good targeting ability. Among them, rhein-loaded nanoparticles have good targeting ability of colon lesion inflammation and colon injury. When the nanoparticle is orally administered, it maintains stability in the stomach and small intestine, releases the drug slowly, and continuously releases the drug after targeting the colon lesion, and has a good effect on treating colitis, especially ulcerative colitis. The nanoparticle has excellent therapeutic effect and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a rhein-loaded lactoferrin nanoparticle and a preparation method and application thereof. Background technique [0002] Ulcerative colitis (UC), a common inflammatory disease of the colon, is increasing in incidence and prevalence worldwide and is more prevalent in younger adults, suggesting that it is a global disease. UC is characterized by disruption of the epithelial barrier and imbalance of inflammatory homeostasis. Therefore, the main goal of clinical UC treatment is to accelerate colonic healing and reduce inflammation. Currently, the first-line therapy for UC mainly focuses on anti-inflammatory drugs, immunomodulators and biological products. However, adverse reactions such as nephrotoxicity, neurotoxicity, and opportunistic infections have been reported in patients using various clinically approved drugs. To improve therapeutic efficacy and re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K31/192A61K47/36A61K47/42A61P1/04A61P29/00
CPCA61K9/5169A61K9/5161A61K31/192A61P1/04A61P29/00Y02A50/30
Inventor 高飞罗锐锋林美斯章津铭廖婉石金凤张晨陈其艳
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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