Preparation method and application of a kind of hydrogel with anisotropic stiffness gradient

Abstract

The invention provides a preparation method and application of anisotropic stiffness gradient hydrogel, including: S1, preparing a polydimethylsiloxane mold in the shape of a lens groove; S2, in the polydimethylsiloxane mold The first preparation raw material is added into the lens groove of the first layer and between the activated first cover glass, and the mold is polymerized and removed to obtain the first layer of hydrogel in the shape of lens groove; S3, in the first layer of hydrogel and hydrophobic The second preparation raw material is added between the second coverslips, and a second layer of hydrogel is obtained by polymerization; S4, the second coverslip is removed to obtain a hydrogel with an anisotropic stiffness gradient. The hydrogel interface provided by the present invention is closer to the interface of the host tissue infected by bacteria, and through the establishment of the physical properties, the mechanical law of bacterial colonization and infection on the hydrogel interface with gradient stiffness can be more effectively revealed.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a preparation method and application of a hydrogel with anisotropic stiffness gradient. Background technique [0002] The colonization and infection of bacteria in the host are regulated by multiple factors, and the research field of their biological characteristics has been widely developed, but the research on the mechanical and physical characteristics still needs to be further explored. The host extracellular matrix (Extracellular matrix, ECM) is an important factor to ensure the integrity and mechanical homeostasis of the host tissue, and its matrix stiffness (Matrix stiffness) is the main mechanical property of the ECM. The polyacrylamide hydrogel technique for simulating different ECM matrix stiffnesses has been vigorously developed, and the matrix stiffness formed on the surface of each hydrogel was measured and prepared by Young's modulus method. The stif...

AI Technical Summary

Problems solved by technology

[0004] In view of the above problems, the present invention provides a preparation method and application of a hydrogel with anisotropic stiffness gradient (gradient change of continuous stiffness value), which is used to at least partially solve the traditional method of using a uniform ECM stiffness hydrogel on bacteria. There are technical problems such as limitations in conducting research on infection

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