Genetic element and using method thereof

Abstract

The present invention provides a genetic element and a using method thereof. The genetic element is used for modulating the expression of a gene, and has a transcription factor sequence disposed upstream of the gene for coupling the transcription factor to the expression of the gene; and a promoter sequence, which is configured at the upstream of the transcription factor sequence and is used for promoting the expression of a downstream sequence of the promoter. The method has the advantages that the method does not depend on any induction conditions including chemical molecules and physical conditions; manual supervision is not needed in the whole process; the gene line is very simple, and is low in cost and high in efficiency when being transformed and deployed to different chassis cellsor applied to different fermentation products.

Description

technical field [0001] The present invention relates to the field of gene regulation, in particular, to a gene element and a method for using the gene element. Background technique [0002] At present, humans have achieved the production of a large amount of energy, chemicals and drugs by converting self-generated endogenous energy and metabolites into pre-designed metabolites through gene editing and other technologies. However, in the process of producing such exogenous products, it will inevitably compete with cells for resources, and even produce toxicity to the cells themselves, resulting in the destruction of the normal structure of the cells or the disorder of the metabolic network. Failure, cell death, reduced yield, waste of energy and carbon sources, etc. [0003] By dynamically regulating the gene expression of cells in the metabolic process, the utilization rate of substrates and the yield of products can be effectively improved, and the dynamic regulation of ce...

AI Technical Summary

Problems solved by technology

[0005] Another type of dynamic regulation strategy is to couple the cell's own metabolic processes, such as transcription factors that respond to intracellular precursor metabolites, through which these transcription factors specifically respond to the concentration of precursor substances in the cell, when the concentration of precursor substances in the cell increases To a certain threshold, the expression of exogenous circuits is turned on. These transcription factors are often endogenous proteins of the cell, so they not only control the transcription of exogenous gene circuits, but also inevitably start the transcription of other metabolic pathways in the cell. On the one hand, it causes competition for intracellular resources, and on the other hand, it will lead to unpredictable metabolic changes; at the same time, coupling exogenous gene circuits and endogenous metabolic pathways involves a large number of trial-and-error editing of the cell genome, making development difficult and long-term

Images