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Analysis method, device and equipment based on 16S microorganism amplification sequencing data

A technology of sequencing data and analysis methods, applied in the field of microbial analysis, can solve problems such as simple analysis process

Pending Publication Date: 2021-03-12
哈尔滨因极科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the library construction and sequencing of experimental samples mainly rely on the Illumina platform, and the analysis of experimental data mainly relies on the bioinformatics analysis of sequencing data. At present, there is no complete analysis method for bioinformatics analysis technology. Even if there is, the analysis process is too simple, resulting in inability to lots of variance analysis

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  • Analysis method, device and equipment based on 16S microorganism amplification sequencing data
  • Analysis method, device and equipment based on 16S microorganism amplification sequencing data
  • Analysis method, device and equipment based on 16S microorganism amplification sequencing data

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Embodiment Construction

[0044] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0045] Such as figure 1 As shown, an analysis method based on 16S microbial amplification sequencing data is proposed, and the analysis method based on 16S microbial amplification sequencing data can be applied to a terminal. This embodiment uses the terminal as an example for illustration. The analysis method based on 16S microbial amplification sequencing data specifically includes the following steps:

[0046] Step 102, obtaining the original sequence data obta...

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Abstract

The embodiment of the invention discloses an analysis method, device and equipment based on 16S microorganism amplification sequencing data; the method comprises the steps: obtaining original sequencedata obtained through the sequencing of 16S microorganisms, and filtering the original sequence data to obtain effective sequence data; determining quality values of a forward sequence and a reversesequence in the effective sequence data; filtering the forward sequence and the reverse sequence according to the quality value to obtain a filtered forward sequence and a filtered reverse sequence; matching and combining the forward sequence and the reverse sequence are and outputting an OTUs sequence after combination; performing comparison annotation on the OTUs sequence and a microorganism database, and determining species annotation and abundance information corresponding to the OTUs sequence; performing clustering grouping according to the species annotation and abundance information corresponding to the OTUs sequence to obtain a plurality of groups; and performing inter-group difference analysis based on the plurality of obtained groups. The analysis method has a complete analysis process and is beneficial to accurate inter-group difference analysis.

Description

technical field [0001] The present invention relates to the technical field of microbial analysis, in particular to an analysis method, device, computer equipment and storage medium based on 16S microbial amplification sequencing data. Background technique [0002] 16S rDNA is located on the small subunit of ribosomes in prokaryotic cells, including 10 conserved regions (Conserved Regions) and 9 hypervariable regions (Hypervariable Regions), among which the conserved regions have little difference among bacteria, and the hypervariable regions have genus or species specificity Sex, there are certain differences with different kinship. Therefore, 16S rDNA can be used to reveal the characteristic nucleic acid sequences of biological species, and is considered to be the most suitable index for bacterial phylogeny and taxonomic identification. [0003] The implementation of 16S (ie 16S rDNA) microbial sequencing technology is mainly divided into two parts, one is the library con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B30/10G16B40/00G16B50/30G06K9/62
CPCG16B30/10G16B40/00G16B50/30G06F18/23
Inventor 刘志岩郭方刘珍郑青松
Owner 哈尔滨因极科技有限公司