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A kind of silver nano hydrogel that regulates flora to activate immune response and its preparation method and application

A hydrogel and silver nanotechnology, applied in the field of biomedicine, can solve the problems of inability to specifically regulate the commensal flora, hinder the effect of immunotherapy, and low responsiveness of malignant tumors, so as to improve host immunity, low equipment requirements, The effect of excellent biocompatibility

Active Publication Date: 2022-04-15
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Under the background of low responsiveness of malignant tumors and impeding the effect of immunotherapy, the purpose of the present invention is to provide a gel product that can be applied to the prevention or treatment of tumors in view of the defects that the current gel products have a single function and cannot specifically regulate the commensal flora. Silver nano hydrogel for regulating bacterial flora to activate immune response and preparation method thereof

Method used

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  • A kind of silver nano hydrogel that regulates flora to activate immune response and its preparation method and application
  • A kind of silver nano hydrogel that regulates flora to activate immune response and its preparation method and application
  • A kind of silver nano hydrogel that regulates flora to activate immune response and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Regulating the Silver Nanohydrate Preparation Method

[0030] Agno 3 The proportion of deionized water was dissolved in deionized water in a proportion of 1: 10 to 5: 1 to form 0.2 to 1 wt% solution. After the UV was irradiated at room temperature, the silver nanoparticles were removed, dispersed in a 0.5 to 2 wt% chitosan solution to obtain a solution A, and the concentration of silver nanoparticles in the solution A in the solution A was 0.2 to 1.2 mg / ml. The glucan is dissolved in deionized water to form 1 to 5 wt% solution, the addition of the glucan mass ratio of 1: 5 ~ 1: 10 4 After stirring for 12 h, a solution B containing 1 to 5 wt% of polymerlyl glucan was formed. Such as figure 2 As shown, the solution A and solution B are mixed between the volume ratios 1: 2 to 3: 1 to obtain a silver nanoferder.

Embodiment 2

[0031] Example 2 Regulating a flora activating an immune response?

[0032] Holled 6-week old female C57BL / 6 mice, governed 4-NQO 100 μg / ml of drinking water for 16 weeks, establishing a mouse oral squamous cell carcinoma in situ model.

[0033] The chitosan of 10 mg of citronomantan dye CY5 and 1G was stirred with 1% by weight of Cy5-chitosan solution overnight. Add 0.68G AGNO in 150ml deionized water3 And 0.35 g of glucan T100, at room temperature UV irradiation, 5000 g of the stirring reaction was centrifuged to obtain AGNP, dispersed in a 1 wt% Cy5-chitosan solution to obtain a solution A, and the concentration of silver nanoparticles in solution A was 0.6 mg / ml. The 1 g of glucan T100 was dissolved in 50 ml of deionized water, add 0.1 g kio 4 After stirring for 12 h, the solution B was formed. The solution A and solution B were mixed with a volume ratio of 1: 2 to obtain a silver nanofeng gel of the cyanocyan dye CY5.

[0034] Silver nanofil gel labeled with Cyanyanide ...

Embodiment 3

[0035] Example 3 Regulating the flora activating immune response to the syndrome effect

[0036] Add 0.57 g of AgNO in 150ml deionized water 3 And 0.25 g of glucan T100, room temperature UV was irradiated, and the reaction was centrifuged after 12 h after 12 h.

[0037] 10 g of urea was added to 5 ml deionized water, and the suspension was placed in alumina crucible. The mixture was heated to 550 ° C, reacted for 3 hours, cooled to 25 ° C, washed three times with deionized water, resulting in c 3 N 4 Nanoparticles.

[0038] 13g FECL 3 · 6h 2 O and 6.5G fecl 2 · 4h 2 O Soluble in 80 ml of deionized water, and the nitrogen gas was filled with 0.5 h in the solution. Add 45 ml NH at 80 ° C. 3 · H 2 O, then 7.5 g of citric acid dissolved with 15 ml of deionized water. 95 ° C reacted 90min, obtained FE 3 O 4 The nanoparticles were purified by dialysis bags (MWCO: 14000Da).

[0039] Cellulose, ZnO, graphene nanoparticles are purchased from the market.

[0040] 100ml 5mm haucl 4 Solution ...

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Abstract

The invention discloses a silver nano hydrogel capable of regulating bacterial flora to activate an immune response, a preparation method and an application thereof, and belongs to the field of biomedicine. The silver nano hydrogel is composed of silver nano particles and mucoadhesive gel. The silver nano hydrogel contains inorganic silver nano particles, natural polymer polysaccharides, polyaldehyde-based polymer materials and deacetylated polymer materials. The silver nanohydrogel can regulate the abundance of mucosal flora, inhibit the proliferation of other bacteria, and at the same time promote the proliferation of immune-activating bacteria such as anaerobic Peptostreptococcus, thereby enhancing the immune response. At the same time, the gel can also adhere to the human mucous membrane, allowing it to stay in the affected area for a long time and continuously regulate the symbiotic flora of the human body. The silver nano hydrogel is biodegradable, has excellent biocompatibility, is safe and has no toxic or side effects, and has the advantages of simple preparation, continuous high efficiency and the like. The invention has great social and economic significance for regulating symbiotic flora, activating human immunity, assisting anti-cancer and other biomedical fields.

Description

Technical field [0001] The present invention belongs to the field of biomedicine, and more particularly to a silver nanofluid gel and preparation method and application thereof for regulating the flora activating immune response. Background technique [0002] Immunotherapy is a treatment of cancer by mobilizing the human immune system. This method has important significance for the treatment of high antigen load tumors, but lower response is the main obstacle to limit immunotherapy to function, up-regulate immunotherapy response rate is one of the important breakthroughs in improving immunotherapy effects. [0003] There is evidence that the spatial position of the microbial flora and certain cancer is highly complicated and is closely related to the effectivity of the host immune system. Bacteria with immunoactive effects can effectively adjust the main immune response strength. It can be seen that the regulatory flora or becomes a way to be a tumor immunougmentary strategy. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K33/38A61K47/36A61K47/38A61P35/00A61P37/04
CPCA61K33/38A61K9/06A61K9/0014A61K47/36A61K47/38A61P35/00A61P37/04
Inventor 张先正郑迪威严雨曾旋
Owner WUHAN UNIV
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