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A method for producing l-lactic acid by utilizing lignocellulose simultaneous saccharification and fermentation

A simultaneous saccharification and fermentation, lignocellulose technology, applied in the field of fermentation engineering, can solve the problems of inconsistent fermentation temperature, environmental pollution process, high production cost, etc., achieve the effect of low cost, prolong fermentation time and increase yield

Active Publication Date: 2022-08-02
上海汉禾生物新材料科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN102174600A discloses a method for continuous fermentation to produce L-lactic acid, and CN102134581A discloses a method for fermentation of lactic acid bacteria, both of which are to process crop waste or plant straw through acid and alkali pretreatment, and use lactic acid bacteria to carry out continuous fermentation or simultaneous fermentation at 40°C. Saccharification and fermentation, patent Although the fermentation cycle has been shortened by optimizing the process, the acid-base pretreatment still has the problems of polluting the environment and complex processes in the later stage
At the same time, the fermentation temperature is inconsistent with the optimum cellulase enzymatic hydrolysis temperature (48°C-50°C), so there is a problem of low enzymatic hydrolysis efficiency in the process of synchronous saccharification
[0007] Therefore, the method of utilizing lignocellulose to produce L-lactic acid also has problems such as pretreatment, environmental pollution, long fermentation cycle and high production cost.

Method used

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  • A method for producing l-lactic acid by utilizing lignocellulose simultaneous saccharification and fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) Bacillus coagulans fermented seed liquid culture: inoculate Bacillus coagulans on a medium plate, place it in a 45°C constant temperature incubator for 12 hours, and connect the grown Bacillus coagulans to the liquid medium, and the culture conditions are temperature 45°C, rotating speed 150rpm, culture time 12h, ventilation rate 1vvm, cultured into Bacillus coagulans seed solution;

[0035] The liquid medium is composed of: glucose 15g / L, yeast powder 5g / L, sodium chloride 2g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.1g / L, manganese sulfate monohydrate 0.02g / L, Ferrous sulfate heptahydrate 0.01g / L;

[0036] (2) Bacillus stearothermophilus fermentation seed liquid culture: inoculate Bacillus stearothermophilus on a medium plate, place it in a 50°C constant temperature incubator for 12 hours, and connect the grown Bacillus stearothermophilus to the liquid medium medium, the culture conditions are the temperature of 50°C, the rotational...

Embodiment 2

[0042] (1) Bacillus coagulans fermented seed liquid culture: inoculate Bacillus coagulans on a medium plate, place it in a constant temperature incubator at 45°C-55°C for 24 hours, connect the grown Bacillus coagulans to the liquid medium, and cultivate The conditions are that the temperature is 55°C, the rotating speed is 250rpm, the incubation time is 24h, and the ventilation rate is 3vvm, and the seed solution of Bacillus coagulans is cultivated;

[0043] The liquid medium is composed of: glucose 25g / L, yeast powder 12g / L, sodium chloride 4g / L, potassium dihydrogen phosphate 3g / L, magnesium sulfate heptahydrate 0.3g / L, manganese sulfate monohydrate 0.07g / L, Ferrous sulfate heptahydrate 0.02g / L;

[0044] (2) Bacillus stearothermophilus fermentation seed liquid culture: inoculate Bacillus stearothermophilus on a medium plate, place it in a 52°C constant temperature incubator for 24 hours, and connect the grown Bacillus stearothermophilus to the liquid medium medium, the cult...

Embodiment 3

[0050] (1) Bacillus coagulans fermented seed liquid culture: inoculate Bacillus coagulans on a medium plate, place it in a 50°C constant temperature incubator for 18 hours, connect the grown Bacillus coagulans to the liquid medium, and the culture conditions are temperature 50°C, rotating speed 200rpm, culture time 18h, ventilation rate 3vvm, cultured into Bacillus coagulans seed solution;

[0051] The liquid medium is composed of: glucose 20g / L, yeast powder 7g / L, sodium chloride 3g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate monohydrate 0.05g / L, Ferrous sulfate heptahydrate 0.015g / L;

[0052] (2) Bacillus stearothermophilus fermentation seed liquid culture: inoculate Bacillus stearothermophilus on a medium plate, place it in a 55°C constant temperature incubator for 18 hours, and connect the grown Bacillus stearothermophilus to the liquid medium medium, the culture conditions are temperature 55°C, rotation speed 200rpm, c...

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Abstract

The present invention proposes a method for producing L-lactic acid by utilizing lignocellulose fermentation and its lactic acid product. Including the following steps: inoculating Bacillus coagulans fermented seed liquid and Bacillus stearothermophilus fermented seed liquid in a fermentation tank containing fermentation medium components, then adding steam explosion pretreated crop material, cellulase and amino acid 1, using alkali The pH was adjusted, and after fermentation at 40-50°C for 6-12h, vitamin B substances and amino acid 2 were added, the temperature was raised to 50-55°C, and the fermentation was continued for 18-48h. The method has the comprehensive advantages of simple process, short fermentation period, high optical purity of lactic acid, high lactic acid yield, easy purification and no pollution in the whole production process, effectively controls the production cost and facilitates industrialization.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a method for producing L-lactic acid by utilizing lignocellulose simultaneous saccharification and fermentation. Background technique [0002] Lactic acid, there are two stereoisomers, namely L-(+) lactic acid and D-(-) lactic acid. As an important biochemical raw material, L-lactic acid is widely used in the fields of food, medicine, health care, modern agriculture and daily chemical industry. In recent years, with the in-depth research on new biological materials, bio-based polymers, especially polylactic acid formed by polymerizing L-lactic acid as a monomer, have attracted much attention. As a biodegradable material, polylactic acid is the most promising environmentally friendly bioplastic, and has the potential to solve the "white pollution" problem caused by petrochemical plastics. [0003] The production methods of lactic acid include chemical method and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12P39/00C12N1/20C12R1/07C12R1/01
CPCC12P7/56C12P39/00C12N1/20C12P2203/00C12P2201/00
Inventor 韩琴涂濂波汤超陶程程
Owner 上海汉禾生物新材料科技有限公司
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