High-throughput measurement device and method for single cell membrane tension

Abstract

The invention discloses a high-throughput measurement device and method for single cell membrane tension. The device comprises a micro-fluidic chip module, a pressure control module, a multi-channel image acquisition module and a data analysis and processing module. The micro-fluidic chip module is used for carrying out cell separation and providing a multi-channel single cell measurement environment; the pressure control module is used for providing negative pressure and driving cells to move through the negative pressure; the multi-channel image acquisition module is used for acquiring cellimages; the data analysis and processing module is used for processing the images acquired by the multi-channel image acquisition module to obtain cell deformation; and based on the cell deformation and the pressure intensity of the negative pressure provided by the pressure control module, the tension of the cell membrane is obtained by adopting a single-cell cell membrane tension equivalent mechanical model. The device can effectively realize high-throughput measurement of the cell membrane tension of the single cell.

Description

technical field [0001] The invention relates to the technical field of cell membrane tension measurement, in particular to a high-throughput measurement device and method for single-cell cell membrane tension. Background technique [0002] Cells are the basic structural and functional units of living organisms. During the life activities of living organisms, they are constantly accompanied by cell proliferation, migration, invasion and differentiation. Since the discovery of cells by British scientist Robert Hooke in 1665, human research on cells has never stopped. It can be said that the state of cells directly or indirectly reflects the state of living organisms. [0003] However, previous cell research methods have focused on populations of cells, resulting in results that are averaged across cell populations, often masking differences that exist between cells. In many cases, data obtained at single-cell resolution more realistically reflect cell state and cell-to-cell ...

AI Technical Summary

Problems solved by technology

In recent years, driven by microfluidic technology, single-cell detection has achieved an increase in detection throughput, but a high-throughput detection method for single-cell epidermal tension has not yet appeared.

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