Pretreatment and determination method for detection of botrytis lipid content by fluorescence spectroscopy

Abstract

The invention relates to the technology for development and utilization of Botryococcus, and aims at providing a pretreatment and a determination method for detecting Botryococcus lipid content by fluorescent spectrometry. The method comprises the following steps of: adding to-be-determined Botryococcus liquid in a centrifuge tube, adding particle type xanthomonas polysaccharide, then, placing the centrifuge tube in water bath, and preparing Botryococcus colony formed by dozens even hundreds of cells into aggregation of uniformly distributed single cells or several cells by using ultrasonic waves, so as to meet the requirement of the fluorescent spectrometry on the uniformity of the determined Botryococcus liquid. The method provided by the invention has the advantages of being low in dosage of Botryococcus liquid, high in sensitivity, excellent in stability, time-saving and labor-saving, capable of achieving high-flux measurement, and so on; and the method can provide necessary technical approaches for research and development of Botryococcus breeding and culturing, and so on.

Description

technical field [0001] The invention belongs to the development and utilization technology of energy microalgae-botrytis, and particularly relates to a pretreatment and measurement method for detecting the lipid content of botrytis by using fluorescence spectroscopy. Background technique [0002] Botryococcus is a kind of energy microalgae widely distributed in the world. It is known as "oil algae" because of its high lipid content (up to 85% of dry cell weight), and its composition and structure are similar to those of fossil crude oil. ", the production of high-quality fuels such as aviation fuel has been one of the research and development hotspots in the global bioenergy field in recent years [Metzger & Largeau. Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Appl Microbiol Biotechnol. 2005, 66: 486–496]. The establishment of fast, trace and reliable lipid content detection technology is an important basis for the research and development ...

AI Technical Summary

Problems solved by technology

This colony-like cell mass is difficult to distribute evenly in the culture medium due to its large size and easy sinking, which seriously affects the feasibility and accuracy of the Nile Red fluorescence method to detect its lipid content.

Because of this, the traditional dry weight method is still used in the research and development of botrytis to detect its lipid content, which is also one of the important reasons for the slow progress in research and development of botrytis breeding, cultivation and deep processing at home and abroad for many years.[ Yaming Ge, et al. Growth characteristics of Botryococcus braunii 765 under high CO 2 concentration in photobioreactor. Bioresource Technology. 2011, 102(1): 130–134]