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A method for detecting the oil content of flowers of aromatic plants

A technology for aromatic plants and flowers is applied in the field of detection of oil content of aromatic plants, which can solve the problems of time-consuming and laborious, large amount of raw materials, and difficulty in meeting the requirements.

Active Publication Date: 2015-09-09
ZHEJIANG UNIV
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Problems solved by technology

Due to the large amount of raw materials used, time-consuming and labor-intensive, this method is difficult to meet the actual needs of breeding new varieties of aromatic plants with high yields of essential oils and developing high-efficiency extraction techniques.

Method used

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Embodiment Construction

[0021] Nile red (9-(diethylamino) benzo[a]phenoxazin-5(5H)-one) is a kind of lipophilic oxazine dye, which can combine with the oil in the flowers of aromatic plants and emit fluorescence at specific wavelengths. Therefore, under the premise of sufficient amount of Nile Red, the lipid content of the tested sample can be characterized by measuring the fluorescence intensity value. In the method of the present invention, the fresh flowers of aromatic plants are fully ground with liquid nitrogen and quartz sand, and then dimethyl sulfoxide, which is known as a "universal solvent", is added, followed by ultrasonication and heating and reflux, the purpose of which is to fully extract the essential oils in the fresh flowers To the solvent, easy to distill out. At the same time, adding dimethyl sulfoxide to the Nile Red dyeing fluorescence detection system is to make the essential oil fully soluble in the aqueous solution, so that the essential oil can be fully combined with Nile Red...

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Abstract

The invention relates to technology of aromatic plant breeding and product deep processing, and aims at providing a method for detecting fresh flower oil content of aromatic plants. The method comprises the following steps: firstly performing full extraction and distillation separation of essential oil in aromatic plant fresh flowers, adding dimethyl sulfoxide to dissolve with the essential oil; detecting a fluorescence emission spectrum between 450 and 700 nm under the excitation of 490-nm photons by using the characteristic that Nile red can bond with aromatic plant fresh flower essential oil and emit fluorescence with a specific wavelength; comparing the obtained result with a blank sample, then comparing with a fluorescence-emission luminous intensity curve of an oil standard substance of triolein so as to obtain the essential oil content in the detected aromatic plant fresh flower sample according to a corresponding luminous intensity value. Compared with traditional weighing methods, the method uses few raw materials, has higher sensitivity and simplicity, saves labor and time, can realize high-flux measurement, and provide a necessary technical method for the research and development of aromatic plant fresh flower breeding, cultivation, and essential oil extraction process.

Description

technical field [0001] The invention relates to aromatic plant breeding and product deep processing technology, in particular to a method for detecting the oil content of aromatic plants. Background technique [0002] Aromatic plants are also called spice plants, aromatic flowers or herbs. Most of them grow in warm areas and have fragrance. Some or all of them can be used in cooking, tea drinking, flower art, crafts, health care, spices, dyeing, medicinal and gardening, etc. Versatile plant. According to incomplete statistics, there are more than 80 families and more than 3,600 kinds of aromatic plants in the world, but only more than 400 kinds have been effectively developed and utilized so far; my country has found that there are more than 60 families and more than 400 kinds of aromatic plants that are valuable for development and utilization. Among them, more than 100 kinds of natural spices have been mass-produced. The aromatic substances of most aromatic plants such as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 汪志平马丽芳于金鑫刘新颖
Owner ZHEJIANG UNIV
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