Composition comprising aster sphathulifolius maxim extract for preventing, improving, or treating muscular diseases or for improving muscular functions
A kind of muscle disease, composition technology, applied in the field of matter
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Embodiment 1
[0110] Embodiment 1: the preparation of sea chrysanthemum extract
[0111] 1-1. Wash the ground part of sea chrysanthemum with water
[0112] Sea chrysanthemum was collected in Jeju Island, Korea, from October to December, and the ground part of sea chrysanthemum was dried at room temperature to make a powder, and the powder that passed through a No. 4 sieve was used. Add 100L of purified water to 10kg of the powder of the ground part of sea chrysanthemum, wash and dehydrate in a cycle at room temperature, and then discard the washing solution. The washing and dehydration treatment was repeated two more times, and at this time, the concentration of chloride ions in the final washing liquid was 10 ppm or less.
[0113] 1-2. Preparation of the ground part extract of sea chrysanthemum
[0114] 100 L of a mixed solvent of water and ethanol at a mixing ratio of water and ethanol (v / v) of 1:10 was added to the ground part powder of the washed sea chrysanthemum, and cooled by reflu...
Embodiment 2
[0115] Example 2: Establishing a Muscle Atrophy Induced Mouse Model and Measuring Muscle Tissue Weight
[0116] Animal experiments were conducted to confirm whether sea chrysanthemum extract improved muscle atrophy in a mouse model of muscle atrophy. Five-week-old male C57BL / 6 mice were acclimatized in the animal laboratory environment for one week, then divided into five groups according to the experimental conditions, and their legs were immobilized to induce muscle atrophy, and samples were orally administered for two weeks. Specifically, the experimental group was divided into an untreated control group (normal group, Cont), a muscle atrophy-inducing group (induction group, IM), muscle atrophy was induced and administered β-hydroxy β-methylbutyric acid (HMB) (370mg / kg) of the positive control group (HMB administration group, HMB 370), inducing muscle atrophy and administering a low dose (100mg / kg) of sea chrysanthemum extract group (sea chrysanthemum low-dose administratio...
Embodiment 3
[0117] Example 3: Confirmation of the effect of reducing the cross-sectional area of muscle fibers by administering sea chrysanthemum extract
[0118] Histological analysis was performed to confirm whether sea chrysanthemum extract ameliorates the reduction of muscle fiber cross-sectional area in a muscular atrophy mouse model. The gastrocnemius muscle and quadriceps muscle tissues extracted in the above-mentioned embodiment 2 were fixed with 4% paraformaldehyde, then hematoxylin and eosin staining (H&E staining) was carried out, and then the cross-sectional area of the stained muscle fiber was carried out by image J software Quantitatively, and the results are as figure 2 and image 3 shown.
[0119] The results confirmed that in both muscle tissues, muscle atrophy was induced to greatly reduce muscle fibers in the induction group (IM) compared with the normal group (Cont), while in the positive control group (HMB 370), sea chrysanthemum The reduction in muscle fiber ...
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