Antibacterial composition and application of antibacterial composition in cleaning products
A composition and fungus technology, applied in the directions of detergent compositions, soap detergent compositions, organic compounds-containing cleaning compositions, etc., can solve problems such as harm to human skin, achieve fine and rich foam, easy washing, and ensure sanitation and health. Effect
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preparation example Construction
[0044] Preparation of medium:
[0045] (1) Preparation of nutrient agar medium: Weigh 3.0g of beef extract, 10.0g of peptone, 15.0g of agar powder, and 5.0g of sodium chloride into a 1000mL beaker, add 1000mL of sterile water to dissolve, boil, and cool to 60°C, adjust the pH value to 7.2-7.4 with 1mol / L sodium hydroxide or 1mol / L hydrochloric acid, dispense into 250mL Erlenmeyer flasks, put on vent stoppers, autoclave at 121°C for 20 minutes, and refrigerate for later use.
[0046] (2) Preparation of nutrient broth medium: Weigh 3.0 g of beef extract, 10.0 g of peptone, and 5.0 g of sodium chloride into a 1000 mL beaker, add 1000 mL of sterile water to dissolve, boil, and cool to 60 ° C. Use 1mol / L sodium hydroxide or 1mol / L hydrochloric acid to adjust the pH value to 7.2-7.4, dispense into 250mL or 500mL Erlenmeyer flasks or 10mL or 25mL test tubes, plug them with ventilating stoppers, autoclave at 121°C for 20 minutes, and refrigerate for later use .
[0047] (3) Staphylo...
experiment example 1
[0058] Exploration of plant antibacterial components and their proportioning ratio in experimental example 1 antibacterial composition
[0059] In order to explore the effective addition amount and optimal ratio of each plant antibacterial component, the following comparative experiments were carried out. The components and proportions of each experimental group are shown in Table 1.
[0060] Staphylococcus aureus, Escherichia coli and Candida albicans were used as test bacteria respectively. Set up experimental groups 1-13: under aseptic operation, draw 1mL of the test bacterial suspension, add 98mL of nutrient broth medium, mix well, and then add 1mL of the phytostatic agents of experimental groups 1-13, Experimental groups 1-13 were obtained, and three parallel experiments were set up in each group to repeat the experiment. Set up a blank control group at the same time: draw 1mL of bacterial propagule suspension, add 98mL of nutrient broth medium, mix well, then add 1mL o...
experiment example 2
[0073] Exploration of the effective antibacterial dosage of experimental example 2 antibacterial composition
[0074] In order to explore the effective bacteriostatic dosage of the bacteriostatic composition, the bacteriostatic composition of the experimental group 10 in Experimental Example 1 was used to conduct the following comparative experiment.
[0075] 1. Experimental group: with sterile water as the diluent, each component is calculated by mass fraction:
[0076] ① 0.5% antibacterial composition: add 99.5% sterile water to 0.5% antibacterial composition;
[0077] ②1% antibacterial composition: add 99% sterile water to 1% antibacterial composition;
[0078] ③ 1.5% antibacterial composition: add 98.5% sterile water to 1.5% antibacterial composition;
[0079] ④ 2% antibacterial composition: add 98% sterile water to 2% antibacterial composition;
[0080] ⑤2.5% antibacterial composition: add 97.5% sterile water to 2.5% antibacterial composition;
[0081] ⑥3% antibacteri...
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