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New drug use of az191

A technology of AZ191, 1.AZ191, applied in the field of new drug use of AZ191, can solve the problem of no AZ191 endogenous cardiomyocyte proliferation and so on

Active Publication Date: 2022-04-05
SYNOGENBIOPHARMACO LTD NANJING CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the induction of endogenous cardiomyocyte proliferation by AZ191.

Method used

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  • New drug use of az191
  • New drug use of az191
  • New drug use of az191

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0047] Test results such as figure 1 shown. Depend on figure 1 It can be seen that after AZ191 treatment, mAG-hGeminin (1 / 110) positive cardiomyocytes increased by 1.8 times compared with the blank control group (0.5% FBS+DMSO). Mean±SEM; **P<0.01. Example 2. In vitro test of AZ191 promoting neonatal rat cardiomyocytes to enter the cell cycle

[0048] Isolate rat cardiomyocytes born 3 days old, add AZ191 (final concentration in the culture medium is 10 μmol / l), use DMEM+DMSO as the control (control), wash three times with PBS after 48 hours, 4% PFA (paraformaldehyde) Fix at room temperature for 15 min, wash with PBS three times, block with 1% BSA / PBS / 0.1% Triton for 1 h at room temperature, add primary antibody after 1 h: Anti-α-Actinin (1:300, sigma, mouse, A7811), Phospho-Histone H3 (Ser10 ) Antibody (1:300, CST, rabbit, 9701S), Anti-Ki67 (1:300, abcam, rabbit, ab15580) overnight at 4°C, wash 4 times with 1% BSA / PBS / 0.02% Tween, add secondary antibody: Goat anti-rabbit ...

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Abstract

The invention discloses a new medicine application of AZ191. The new use is the application of AZ191 or a pharmaceutically acceptable salt thereof in the preparation of drugs for promoting cardiomyocyte proliferation. The present invention proves through experiments that AZ191 can promote the proliferation of rat cardiomyocytes. Therefore, AZ191 can be used in related fields such as the preparation of drugs for promoting cardiomyocyte proliferation and drugs for treating or preventing heart disease, providing a new drug and treatment idea for treating or preventing heart disease such as myocardial infarction.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a new drug application of AZ191. Background technique [0002] At present, cardiovascular disease has become the number one killer threatening human health. There are 40 million heart failure patients worldwide, and it has become the main cause of human death. Studies have found that cardiomyocytes in mammals gradually lose their ability to proliferate after adulthood. Once myocardial infarction occurs, the loss of cardiomyocytes will be irreversible. There are about 2-4 billion cardiomyocytes in an adult, about 25% of cardiomyocytes will be lost within a few hours after myocardial infarction, and the remaining cardiomyocytes have very limited proliferation ability, which is not enough to restore the contractile function of the heart, and the patient will eventually suffer from heart failure die. To solve this problem, in addition to surgery, basic translational research mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/506A61P9/10A61P9/00A61P9/04
CPCA61K31/506A61P9/10A61P9/00A61P9/04
Inventor 卜晔杜建勇郑丽霞吴青朱小君熊敬维
Owner SYNOGENBIOPHARMACO LTD NANJING CHINA