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Small peptide capable of inhibiting metastasis of liver cancer and its preparation method and application

A technology of small molecular peptides and genes, which is applied in the field of small peptides with the function of inhibiting liver cancer metastasis and its preparation, can solve problems such as no high-value applications, and achieve high ability to inhibit the migration of liver cancer cells and inhibit the invasion and metastasis of liver cancer cells. Effect

Active Publication Date: 2022-03-29
SHENZHEN HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

pri-miR-122 is stable and abundant in normal hepatocytes but does not have any high-value applications

Method used

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  • Small peptide capable of inhibiting metastasis of liver cancer and its preparation method and application
  • Small peptide capable of inhibiting metastasis of liver cancer and its preparation method and application
  • Small peptide capable of inhibiting metastasis of liver cancer and its preparation method and application

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Embodiment Construction

[0072] In order to make the invention purpose, technical solutions and technical effects of the present invention clearer, the present invention will be further described in detail below with reference to the specific embodiments. It should be understood that the specific embodiments described in this specification are only for explaining the present invention, rather than for limiting the present invention.

[0073] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial sources.

[0074] Translational validation assay for pri-miR-122

[0075] Separation principle of polysomes in cells by sucrose density gradient centrifugation:

[0076] The higher the number of ribosomes bound to an RNA, the faster the RNA will settle during gradient centrifugation, so that RNAs with different numbers of ribosomes bound can be separated in solution by centrifugation. Using qPCR to detect the co...

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Abstract

The invention discloses a small peptide with the function of inhibiting liver cancer metastasis and its preparation method and application, wherein the small peptide has the amino acid sequence shown in SEQ ID NO.1 to 4 or any one of SEQ ID NO.1 to 4 The shown amino acid sequence is substituted, deleted and / or added with one or more amino acids and / or terminal modified and has the function of inhibiting liver cancer metastasis. The invention also provides a preparation method of the small peptide, and obtains the amino acid site that mediates its rapid degradation. The small peptide in the present invention is found to have the function of inhibiting the invasion and metastasis of liver cancer cells, has a strong ability to inhibit the migration of liver cancer cells, and has the application potential of developing new anti-metastasis drugs or other preparations of liver cancer cells.

Description

technical field [0001] The invention belongs to the field of biologically active peptides, and in particular relates to a small peptide with the function of inhibiting the metastasis of liver cancer and a preparation method and application thereof. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma for short) is one of the most common malignant tumors with high morbidity and mortality in the world. It causes more than 600,000 deaths every year, seriously endangering people's lives and health. Most liver cancer patients have already entered the advanced stage of liver cancer at the time of diagnosis, and may have metastasized. The available treatment methods are limited and the prognosis is poor. For patients with early stage liver cancer, surgical resection is currently the most important treatment method, but its prognosis is not good, and it is easy to recur and metastasize after surgery, resulting in the death of liver cancer patients. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/11C12N5/10A61K38/16A61P35/00A61P35/04
CPCC07K14/4703A61P35/00A61P35/04A61K38/00
Inventor 曾春贤黄宁
Owner SHENZHEN HOSPITAL OF SOUTHERN MEDICAL UNIV
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