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Biomarker for lung adenocarcinoma diagnosis and application of biomarker

A biomarker, lung adenocarcinoma technology, applied in the field of biomedicine, which can solve problems such as expression differences

Active Publication Date: 2021-03-26
XUZHOU CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] By studying a certain amount of clinical lung adenocarcinoma samples, the present invention found that ENSG00000277534 was significantly highly expressed in lung adenocarcinoma tissue, and there was a significant difference compared with the expression of ENSG00000277534 in normal tissue, and then proved by verification experiments ENSG00000277534 can be used as a biomarker for the diagnosis and treatment of lung adenocarcinoma, and has good clinical application value. There is no report on the relationship between ENSG00000277534 and lung adenocarcinoma

Method used

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  • Biomarker for lung adenocarcinoma diagnosis and application of biomarker
  • Biomarker for lung adenocarcinoma diagnosis and application of biomarker
  • Biomarker for lung adenocarcinoma diagnosis and application of biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Differential expression of ENSG00000277534 in lung adenocarcinoma

[0077] 1. Sample source

[0078] Collect 54 pairs of lung cancer tissues from LUAD patients admitted to the hospital and adjacent normal tissues > 3 cm from the tumor edge. All specimens were placed in cryopreservation tubes with RNA protection solution immediately after isolation, and stored at -80°C within 30 minutes. Store frozen in the refrigerator, and the entire operation and storage process follows the principle of no enzymes. All cases were confirmed as lung adenocarcinoma by the pathology department, and none of the patients received special treatment such as radiotherapy, chemotherapy, targeted therapy, and immunotherapy before operation. At the same time, the clinicopathological data of the patients were recorded in detail. The age of the patients ranged from 41 to 75 years, with an average age of 51 years. There were 27 cases with primary tumor ≤ 3 cm, 19 cases with lymph node me...

Embodiment 2

[0129] Example 2 Effect of ENSG00000277534 Expression on Proliferation of Lung Adenocarcinoma Cells

[0130] 1. Cell culture

[0131] The pre-preserved lung adenocarcinoma cell line Calu-3 was cultured, and the cell culture conditions were: 37°C, 5% CO 2 , DMEM (Hyclone) culture medium, 10% fetal bovine serum (Sijiqing), 1% double antibody (100 μg / mL streptomycin, 100 Units / mL penicillin).

[0132] 2. Cell transfection

[0133] 1 day before transfection, Calu-3 cells were seeded in 24-well cell culture plates at 3000 cells / well, and when the cell confluence reached 30%-50%, the TM 3000 instructions transfected cells. Cells were divided into 3 groups: blank control group (no special treatment), siRNA-NC negative control group (transfection of blank plasmid) and siRNA experimental group (stable transfection of siRNA that interferes with the expression of ENSG00000277534), in which, the negative control group siRNA- NC has no homology with the sequence of ENSG00000277534 gene...

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Abstract

The invention discloses a biomarker for lung adenocarcinoma diagnosis and application of the biomarker, and particularly relates to an ENSG00000277534 biomarker for lung adenocarcinoma diagnosis, andexperimental research proves that the expression level of the ENSG00000277534 in lung adenocarcinoma tissues is remarkably increased compared with that of normal control tissue. According to the properties of the ENSG00000277534, a product for diagnosing lung adenocarcinoma can be prepared according to the ENSG00000277534; and in-vitro cell experiments prove that lung adenocarcinoma cell proliferation can be inhibited and cell apoptosis can be promoted by inhibiting the expression of the ENSG00000277534, so that the inhibition of the expression of the ENSG00000277534 can be used as a new treatment strategy for treating lung adenocarcinoma and has good clinical application value.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a biomarker ENSG00000277534 for lung adenocarcinoma diagnosis and its application. Background technique [0002] Lung cancer (Lung cancer, LC) is divided into small cell lung cancer (Small cell lung cancer, SCLC) and non-small cell lung cancer (Non-small cell lung cancer, NSCLC). 85%, mainly including lung adenocarcinoma (Lung adenocarcinoma, LUAD), lung squamous cell carcinoma (Lung squamous cellcarcinoma, LUSC) and so on. Lung cancer is a highly malignant respiratory tumor with the highest mortality rate in the world. In my country, the morbidity and mortality of lung cancer are both the first. The annual number of lung cancer cases exceeds 700,000, and the number of deaths exceeds 600,000. Among them, lung adenocarcinoma accounts for about Half of all lung cancers, many patients are already in the middle and late stages when they are diagnosed. Statistics show that lung ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886A61K45/00A61K31/713A61P35/00A61P11/00
CPCA61K31/713A61K45/00A61P11/00A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158
Inventor 吕茜刘颖高志康陈碧
Owner XUZHOU CENT HOSPITAL