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New strain of pseudoxanthomonas beigongshangensis and application of new strain

A Pseudoxanthomonas, industrial and commercial technology, applied in the field of wine-making microorganisms, can solve the problems of undiscovered microbial species

Pending Publication Date: 2021-03-30
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The microorganisms cultivated by traditional methods are all known microorganisms, and no new microbial species have been discovered

Method used

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  • New strain of pseudoxanthomonas beigongshangensis and application of new strain
  • New strain of pseudoxanthomonas beigongshangensis and application of new strain
  • New strain of pseudoxanthomonas beigongshangensis and application of new strain

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Embodiment 1

[0025] Example 1, the isolation and identification of Pseudoxanthomonas beigongshangensis

[0026] 1. Isolation of new strains of Pseudoxanthomonas beigongshangensis

[0027] Step 1. Configure R2A medium: yeast extract powder 0.5g, sodium pyruvate 0.3g, peptone 0.5g, anhydrous magnesium sulfate 0.024g, glucose 0.5g, dipotassium hydrogen phosphate 0.3g, soluble starch 0.5g, casein hydrolysis Material 0.5g, agar 15g;

[0028] Step 2. Take 10g of pit mud, which comes from Sichuan Luzhou-flavor winery, and dilute it to 10 with sterile distilled water. -5 , spread on the configured R2A medium culture dish at 28°C for aerobic culture;

[0029] Step 3: Pick a single colony after aerobic culture at 28°C for 36 hours, and purify it 3 times in three divisions to obtain pure cultured bacteria;

[0030] Step 4: Extract purely cultured bacterial DNA, amplify the 16SrDNA sequence by PCR technology, and preliminarily determine the new strain of Pseudomonas spp. by sequence comparison.

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Abstract

The invention discloses a new strain of pseudoxanthomonas beigongshangensis and an application of the new strain. The strain is pseudoxanthomonas beigongshangensis, and the preservation number of thepseudoxanthomonas beigongshangensis in the China General Microbiological Culture Collection Center is CGMCC NO.19208. The tests prove that the invention provides a new strain of pseudoxanthomonas beigongshangensis, and the strain is separated from pit mud of white wine, can generate aromatic flavor substances, and can be used for fermenting and brewing wine.

Description

technical field [0001] The invention relates to the technical field of wine-making microorganisms, in particular to a new strain of Pseudomonas spp. and its application. Background technique [0002] Baijiu is one of the three major distilled spirits in the world. It is produced by unique fermentation technology and has a history of thousands of years. The entire inner wall of the pit pool is covered with pre-cultivated pit mud. The fermented raw materials are cooked for blending, crushing and distillation. A saccharification starter is added to the steamed raw materials, and then put into the cellar for fermentation, and the fermented material is taken out of the cellar and distilled to make Chinese liquor. Microorganisms in pit mud produce various flavor components such as butyric acid, caproic acid and ethyl caproate. Ethyl hexanoate is considered to be a key component affecting the flavor and quality of Luzhou-flavor liquor. During the brewing process, cellar mud mic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12G3/02C12R1/01
CPCC12N1/20C12N1/02C12G3/02
Inventor 任清徐嘉良孙占斌闫怡孙乐平邢旋陈海燕国立引
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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