New strain of pseudoxanthomonas beigongshangensis and application of new strain
A Pseudoxanthomonas, industrial and commercial technology, applied in the field of wine-making microorganisms, can solve the problems of undiscovered microbial species
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[0025] Example 1, the isolation and identification of Pseudoxanthomonas beigongshangensis
[0026] 1. Isolation of new strains of Pseudoxanthomonas beigongshangensis
[0027] Step 1. Configure R2A medium: yeast extract powder 0.5g, sodium pyruvate 0.3g, peptone 0.5g, anhydrous magnesium sulfate 0.024g, glucose 0.5g, dipotassium hydrogen phosphate 0.3g, soluble starch 0.5g, casein hydrolysis Material 0.5g, agar 15g;
[0028] Step 2. Take 10g of pit mud, which comes from Sichuan Luzhou-flavor winery, and dilute it to 10 with sterile distilled water. -5 , spread on the configured R2A medium culture dish at 28°C for aerobic culture;
[0029] Step 3: Pick a single colony after aerobic culture at 28°C for 36 hours, and purify it 3 times in three divisions to obtain pure cultured bacteria;
[0030] Step 4: Extract purely cultured bacterial DNA, amplify the 16SrDNA sequence by PCR technology, and preliminarily determine the new strain of Pseudomonas spp. by sequence comparison.
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