Nucleic acid sample preserving fluid as well as preparation method and application thereof

A nucleic acid sample and preservation solution technology, applied in the field of biomedicine, can solve the problems of non-degradation of nucleic acid, secondary transmission, and inability to inactivate viruses in time

Pending Publication Date: 2021-04-02
山东科硕生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For highly infectious viruses, due to the easy degradation of nucleic acid itself, the inventors found that the existing conventional sampling and preservation solutions cannot ensure that the nucleic acid in the sample does not degrade, and at t...

Method used

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  • Nucleic acid sample preserving fluid as well as preparation method and application thereof
  • Nucleic acid sample preserving fluid as well as preparation method and application thereof
  • Nucleic acid sample preserving fluid as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0030] In yet another specific embodiment of the present invention, a method for preparing the above-mentioned nucleic acid sample preservation solution is provided, and the preparation method includes: mixing the above-mentioned components.

[0031] In yet another specific embodiment of the present invention, the application of the above nucleic acid sample preservation solution in in vitro nucleic acid sample preservation is provided. The nucleic acid sample preservation solution prepared by the present invention is suitable for the preservation of throat swabs, nasal swabs or tissue samples of specific parts after sampling, and the stored samples can be used for subsequent clinical experiments such as nucleic acid extraction or purification. The nucleic acid includes ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). It is especially suitable for the preservation of virus (such as RNA virus) nucleic acid samples.

Embodiment

[0034] A nucleic acid sample preservation solution, which consists of the following components:

[0035] 0.3M sodium acetate, 5mM EDTA sodium salt, 5mM Tris, 1μg / ml proteinase K, 0.2% SDS, 0.2% TritonX-100, 0.5% SLS, the solvent is DEPC triple distilled water.

[0036] When using, mix the ingredients and serve.

[0037] Effect verification

[0038] Cell Culture and Subculture

[0039] The HL-60 cells were taken out from the liquid nitrogen bottle and put into a 37°C constant temperature water bath to melt rapidly. The thawed cells were added to three centrifuge tubes (4-5 ml cell culture medium was added to the three centrifuge tubes in advance), and centrifuged at 800 rpm for 3 minutes. Discard the supernatant, add 4-5ml of cell culture medium to each centrifuge tube again, pipette the cell pellet evenly with a pipette, transfer the three types of cells to cell culture flasks or cell culture dishes, and store them at 37°C, 5% CO2 Cultivated in an incubator.

[0040] Cell...

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PUM

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Abstract

The invention provides a nucleic acid sample preserving fluid as well as a preparation method and application thereof, and belongs to the technical field of biomedicine. The nucleic acid sample preserving fluid is composed of sodium acetate, EDTA sodium salt, Tris, protease K, SDS, Triton X-100, SLS and DEPC triple distilled water. According to the invention, a collected sample is fully mixed witha virus lysis solution and a viral nucleic acid preserving fluid, so that the virus in the sample is inactivated, the integrity of viral nucleic acid in the sample is effectively ensured, and the collected sample can be transported and preserved for a long time under a normal temperature condition. The nucleic acid can be released by instantly cracking pathogens, and the nucleic acid can be prevented from being degraded by a protective agent, so that the nucleic acid sample preserving fluid has good practical production and application value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a nucleic acid sample preservation solution and a preparation method and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] A virus is a microorganism with a relatively simple structure. Its method to complete the process of self-replication requires the help of host cells and the nutrition of the host to complete virus replication. There are many common viruses that cause human infection, which can cause respiratory diseases, such as cold virus, influenza virus, new coronavirus and SARS and other coronaviruses. In the process of clinical tes...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/125C12Q2521/537
Inventor 姜国胜高伟王希娣张丹凤任海山陈梅英
Owner 山东科硕生物技术有限公司
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