Tissue culture method capable of increasing quantity of garlic growing point differentiated adventitious buds

A growing point and garlic technology is applied in the field of tissue culture for increasing the number of differentiated adventitious buds at the growing point of garlic, and can solve the problems that it is difficult to satisfy the number of virus-free seedlings, the induction of adventitious buds is low, the reproduction coefficient is low, and the cost is low and the reproduction is low. The effect of high coefficient and simple operation

Inactive Publication Date: 2021-04-06
江苏丰收大地种业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this approach has the disadvantages of low adventitious bud induction, low reproduction coefficient, and high cost during the garlic tissue culture process, and it is difficult to meet the demand for the number of virus-free seedlings.

Method used

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  • Tissue culture method capable of increasing quantity of garlic growing point differentiated adventitious buds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Embodiment 1: as figure 1 As shown, the tissue culture method for improving the number of differentiated adventitious buds of the growth point of garlic specifically includes the following steps:

[0034] S1 disinfection: the selected garlic cloves are pretreated and then sterilized by alcohol and mercuric chloride to obtain sterilized garlic cloves. The step S1 is specifically:

[0035] S11 pretreatment: select robust garlic heads that need to be detoxified, peel off garlic cloves, soak in a solution of 50% carbendazim wettable powder 500 times diluted and added with a concentration of 10ppm tetramycin for 5 hours, then rinse with tap water for 2 hours, and then dry for later use;

[0036] S12 Surface disinfection of garlic cloves: soak the taken garlic cloves in 75% alcohol for 60 seconds, then take them out and rinse them with sterile water for 5 times, then soak them in 0.1% HgCl disinfection solution for 30 minutes, put them in sterile water after taking them out, ...

Embodiment 2

[0043] Embodiment 2: as figure 1 As shown, the tissue culture method for improving the number of differentiated adventitious buds of the growth point of garlic specifically includes the following steps:

[0044] S1 disinfection: the selected garlic cloves are pretreated and then sterilized by alcohol and mercuric chloride to obtain explants. The step S1 is specifically:

[0045] S11 pretreatment: select robust garlic heads that need to be detoxified, peel off garlic cloves, soak in a solution of 500-fold dilution of 50% carbendazim wettable powder and add 10ppm tetramycin for 4 hours, then rinse with tap water for 1 hour, and then dry for later use;

[0046] S12 Disinfection of the surface of garlic cloves: soak the taken explants in 75% alcohol for 70 seconds, then take them out and rinse them with sterile water for 4 to 5 times, then soak them in 0.1% HgCl disinfection solution for 40 minutes, take them out and put them in sterile water , while using sterile running water ...

Embodiment 3

[0054] Embodiment 3: as figure 1 As shown, the tissue culture method for improving the number of differentiated adventitious buds of the growth point of garlic specifically includes the following steps:

[0055] S1 disinfection: the selected garlic cloves are pretreated and then sterilized by alcohol and mercuric chloride to obtain explants. The step S1 is specifically:

[0056] S11 pretreatment: select robust garlic heads that need to be detoxified, peel off garlic cloves, soak in a solution of 500% carbendazim wettable powder diluted 500 times and add tetramycin with a concentration of 10ppm for 6 hours, then rinse with tap water for 3 hours, and then Dried as explants, set aside;

[0057] S12 Disinfection of explant surface: soak the collected explant in 75% alcohol for 45s, then take it out and rinse it with sterile water for 4 to 5 times, then put it into 0.1% HgCl disinfection solution and soak it for 20min, take it out and put it in sterile water. In bacterial water, ...

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Abstract

The invention discloses a tissue culture method capable of increasing a quantity of garlic growing point differentiated adventitious buds. The method specifically comprises the following steps that S1, disinfection is performed: selected garlic cloves are pretreated, then are subjected to disinfecting with alcohol and mercuric chloride in sequence, and 0.5-1.0 mm tissues are stripped at the top ends of stem tips to obtain explants for later use; S2, a culture medium is prepared: an induction culture medium is prepared for later use; S3, the explants are grafted: the explants are grafted into the induction culture medium, and induction culture is performed under the conditions of sterile culture and illumination to obtain stem tip meristem seedlings; and S4, subculture and induced differentiation are performed: after new garlic leaves grow from the adventitious buds, subculture is performed, differentiation and growth of the adventitious buds are induced, and finally bud clusters are differentiated. The method is simple to operate, and under the same culture medium culture and culture conditions, only new leaves need to be stripped continuously, and a stem tip meristem is damaged and stimulated; meanwhile, the propagation coefficient is high and can generally reach 4-5 or higher; and a large quantity of adventitious buds are induced in one step, the time is short, the efficiency is high, and the production cost is effectively reduced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture method for increasing the number of differentiated adventitious buds at growth points of garlic. Background technique [0002] Tissue culture technology is the most important technology to solve the problem of crop detoxification and rejuvenation and rapid seedling production. [0003] Garlic is a perennial herb of the Liliaceae Allium genus. It is a very important fragrant vegetable and condiment. Garlic is produced by asexual reproduction of bulbs. During the long-term asexual reproduction, it is susceptible to various viruses. The viruses mainly include garlic mosaic virus, onion yellow dwarf virus, garlic latent virus, garlic chlorotic streak virus, etc. The incidence rate in the field is as high as 100%, and the phenomenon of bulb degradation is serious, resulting in decreased yield and poor quality. Seriously affect garlic production. Cultiva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 周志疆陈雯雯张桢杨佩娟庄锦贵
Owner 江苏丰收大地种业发展有限公司
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