Nano-enzyme with deep penetrating and multiple tumor killing functions and preparation method and application thereof
A nano-enzyme, multiple technology, applied in the field of biomedicine, can solve the problems of poor targeting and inefficient penetration of ultrasound-activated sonosensitizers
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experiment example 1
[0026] Experimental Example 1: Detect the basic physical and chemical properties of the nanoparticles, including particle size, potential, morphology, ultraviolet absorption spectrum, and encapsulation efficiency.
[0027] The nanoparticles are dark brown emulsion with uniform size and good dispersion. The particle size is 284nm, the measured average potential is -14.7mV, and the measured encapsulation efficiency of IR780, HMME is 68.99±6.02% and 78.31±2.16%, and the encapsulation efficiency of GOX is 7.74±1.9%
experiment example 2
[0028] Experimental example 2: Detection of active oxygen and enzyme activity ( Figure 4 and Figure 5 )
[0029] Detect the basic physical and chemical properties of the nanoparticles, including the characteristics of reactive oxygen species generation, enzyme activity, etc., and observe the ability of the nanoparticles to generate reactive oxygen species (Reactive oxygen species, ROS) in human breast cancer cell line 4T1 cells. And the specific process of the experiment on the ability to generate reactive oxygen species in the cells, etc., as well as the interpretation of the experimental pictures (what dyes are used, the test objects and other information need to be disclosed).
[0030] After ultrasonic irradiation, the ROS production of nanoparticles increased significantly, and the ROS production increased with the increase of irradiation time. GOX on nanoparticles can catalyze glucose to produce gluconic acid and H 2 o 2 , and H2O2 can react with TMB to detect the a...
experiment example 3
[0031] Experimental example 3: Imaging ability detection ( Figure 6 and Figure 7 )
[0032] In vitro photoacoustic imaging capability and in vivo photoacoustic imaging capability were tested.
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