Preparation method and application of composite nanometer material for combined treatment of tumor by optothermal and active oxygen

A composite nanomaterial, combined treatment technology, applied in the direction of photodynamic therapy, antitumor drugs, wave energy or particle radiation treatment materials, etc., to achieve obvious therapeutic effect, simple preparation process and low cost.

Active Publication Date: 2018-07-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the combination of nano-iron / polydopamine / glucose oxidase to construct composite materials and combine photothermal and active oxygen to treat tumors.

Method used

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  • Preparation method and application of composite nanometer material for combined treatment of tumor by optothermal and active oxygen
  • Preparation method and application of composite nanometer material for combined treatment of tumor by optothermal and active oxygen
  • Preparation method and application of composite nanometer material for combined treatment of tumor by optothermal and active oxygen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of the composite nanomaterial of embodiment 1, nano-iron / PDA / GOx

[0034] (1) Preparation of nano-iron matrix

[0035] Weigh 1.35g of FeCl 3 ·6H 2 O was dissolved in 40 mL of ethylene glycol and magnetically stirred until FeCl 3 ·6H 2 O is completely dissolved; add 3.60 g of anhydrous sodium acetate, stir for 30 minutes until the sodium acetate is completely dissolved; pour the solution into a high-pressure reactor lined with polytetrafluoroethylene, set the temperature at 200 ° C, and react in a constant temperature oil bath for 8 hours, then take out After the reaction kettle was cooled to room temperature, it was washed 6 times with methanol, put into a vacuum drying oven to dry overnight, and the TEM image was detected as follows: figure 1 As shown in (a), the particle diameter is 200 nm.

[0036] (2) Preparation of nano-iron / PDA / GOx composites

[0037] Nano-iron is coated with polydopamine by self-assembly method, and 50 mg of nano-iron powder p...

Embodiment 2

[0039] Embodiment 2, experiment of light-to-heat conversion performance of nano-iron / PDA / GOx composite nanomaterial

[0040] (1) Configuration of the reaction system

[0041] Weigh 5 mg of the nano-iron / PDA / GOx composite prepared in Example 1, disperse in 5 mL of phosphate (PBS) buffer, and serially dilute to obtain 10 μg / mL, 100 μg / mL and 1000 μg / mL suspensions. In the experimental group, 200 μL of composite material suspensions were added to a single well of a 96-well plate; in the control group, 200 μL of PBS buffer was added to a single well of a 96-well plate.

[0042] (2) Test of light-to-heat conversion performance

[0043] The experimental group and the control group were respectively exposed to infrared light 3.6W / cm 2 Irradiate for 5 minutes under the same conditions, and use an electronic thermometer to measure and record the temperature change every 30 seconds. The results are as follows: figure 2 As shown, the higher the concentration of nano-Fe / PDA / GOx compos...

Embodiment 3

[0044] Example 3 The specific killing of breast cancer cell MDA-MB-231 by nano-iron / PDA / GOx composite nanomaterial

[0045] The activity of the cells was detected by the MTT method, and the specific steps were as follows:

[0046] (1) Culture breast normal epithelial cells MCF-10A and breast cancer cells MDA-MB-231, culture the cells to 70% confluence, digest, collect and count, and dilute the cell density to 1×10 5 cells / mL, add 100 μL of the above cell dilution solution to each well of a 96-well plate, and place in a 37°C, 5% CO2 incubator for 24 hours;

[0047] (2) Replace with new complete medium, add nano-iron / PDA / GOx composite nanomaterials to a final concentration of 10 μg / mL, 20 μg / mL, 50 μg / mL, 100 μg / mL, 1000 μg / mL, 37 ° C, 5% CO2 Cultivate in the incubator for 4 hours;

[0048] (3) Near infrared light (3.6W / cm2) emitted by BST808-5-F laser for each hole 2 ) after 3min irradiation, 37℃, 5%CO 2 Cultivate in the incubator for 12h;

[0049] (4) Stain the 96-well pl...

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Abstract

The invention relates to a preparation method and an application of a composite nanometer material for the combined treatment of tumor by optothermal and active oxygen, and belongs to the technical field of medicines used for treatment. Nanometer iron is prepared through an ethylene glycol method, a catechol group on a dopamine molecular is oxidized under the condition of alkaline solution and oxygen, dyhydroxy benzpyrole or a semiquinone free radical is formed to generate rearrangement or coupling crosslinking auto polymerization reaction, a polydopamine layer coats the outer surface of the nanometer iron, the active group of the polydopamine is used for realizing covalent linkage with glucose oxidase to prepare a nanometer iron / polydopamine / glucose oxidase (nanometer iron / PDA(propane diamine) / GOx(gaseous oxygen)) composite nanometer material, and the composite material of the inorganic nanometer material modified by biomacromolecules and biomimetic materials is constructed. The prepared material has the advantages of obvious treatment effect, high biocompatibility, no toxicity and no pollution. The preparation technology has the advantages of simpleness and low cost and is suitable for scale production and clinical medical care.

Description

technical field [0001] The invention relates to a preparation method and application of a composite nanomaterial for combined treatment of tumors with photothermal and active oxygen, and belongs to the technical field of therapeutic drugs. Background technique [0002] In cancer treatment, in order to improve the anti-tumor effect and reduce the side effects, it is an important strategy for cancer treatment to develop compound therapeutic agents targeting the weakness of tumor tissue. Tumor cells are very dependent on glucose due to their rapid growth and proliferation. Moreover, due to its unique tissue structure and metabolic characteristics, tumors are much less resistant to heat (>42°C) and exogenous reactive oxygen species than normal cells; Macromolecules and nanomaterials with a certain particle size (100-200nm) have significant retention and accumulation effects (enhanced permeability and retention effect, EPR). [0003] Photothermal therapy is a minimally invas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61P35/00
CPCA61K41/0052A61K41/0057A61K2300/00
Inventor 徐莉张甜甜肖敏李银萍洪伟英
Owner SHANDONG UNIV
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