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Preparation method and application of a composite nanomaterial for combined treatment of tumors with photothermal and active oxygen

A technology of composite nanomaterials and combined therapy, which is applied in the direction of photodynamic therapy, antineoplastic drugs, wave energy or particle radiation treatment materials, etc., to achieve the effect of simple preparation process, low cost and obvious therapeutic effect

Active Publication Date: 2021-02-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the combination of nano-iron / polydopamine / glucose oxidase to construct composite materials and combine photothermal and active oxygen to treat tumors.

Method used

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  • Preparation method and application of a composite nanomaterial for combined treatment of tumors with photothermal and active oxygen
  • Preparation method and application of a composite nanomaterial for combined treatment of tumors with photothermal and active oxygen
  • Preparation method and application of a composite nanomaterial for combined treatment of tumors with photothermal and active oxygen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Preparation of nano-iron / PDA / GOx composite nanomaterials

[0034] (1) Preparation of nano-iron matrix

[0035] Weigh 1.35g of FeCl 3 ·6H 2 O was dissolved in 40 mL of ethylene glycol and magnetically stirred until FeCl 3 ·6H 2 O was completely dissolved; 3.60 g of anhydrous sodium acetate was added, and stirred for 30 minutes until the sodium acetate was completely dissolved; the solution was poured into an autoclave lined with polytetrafluoroethylene, the temperature was set at 200 °C, and after 8 hours of reaction in a constant temperature oil bath, take out After the reaction kettle was cooled to room temperature, it was washed 6 times with methanol, put into a vacuum drying oven for overnight drying, and the TEM image was detected as follows: figure 1 As shown in (a), the particle size is 200 nm.

[0036] (2) Preparation of nano-iron / PDA / GOx composites

[0037] Nano-iron is coated with polydopamine by self-assembly method, and 50 mg of the nano-iron p...

Embodiment 2

[0039] Example 2. Photothermal conversion performance experiment of nano-iron / PDA / GOx composite nanomaterials

[0040] (1) Configuration of the reaction system

[0041] Weigh 5 mg of the nano-iron / PDA / GOx composite prepared in Example 1, disperse it in 5 mL of phosphate (PBS) buffer, and dilute to obtain 10 μg / mL, 100 μg / mL and 1000 μg / mL suspensions. In the experimental group, a suspension of the composite material with a concentration of more than 200 μL was added to a single well of a 96-well plate; in the control group, 200 μL of PBS buffer was added into a single well of a 96-well plate.

[0042] (2) Test of photothermal conversion performance

[0043] The experimental group and the control group were exposed to infrared light of 3.6W / cm 2 Irradiate for 5 minutes under the condition, and use an electronic thermometer to measure and record the temperature change every 30 seconds. The results are as follows figure 2 As shown, the higher the concentration of the nanoiron...

Embodiment 3

[0044] Example 3 Specific killing of breast cancer cells MDA-MB-231 by nano-iron / PDA / GOx composite nanomaterials

[0045] The cell viability was detected by MTT method, and the specific steps were as follows:

[0046] (1) Cultivate normal breast epithelial cells MCF-10A and breast cancer cells MDA-MB-231, culture the cells to 70% confluence, digest, collect and count, and dilute the cells to a density of 1 × 10 5 cells / mL, add 100 μL of the above cell dilution to each well of a 96-well plate, and place it in a 37°C, 5% CO2 incubator for 24 hours;

[0047] (2) Replace the new complete medium and add nano-iron / PDA / GOx composite nanomaterials to the final concentration of 10 μg / mL, 20 μg / mL, 50 μg / mL, 100 μg / mL, 1000 μg / mL, 37°C, 5% CO2 4h in the incubator;

[0048] (3) Near-infrared light (3.6W / cm) emitted by BST808-5-F laser per hole 2 ) after 3min irradiation, 37℃, 5%CO 2 12h in the incubator;

[0049] (4) Stain the 96-well plate, add 10 μL MTT (5 mg / mL) to each well of t...

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Abstract

The invention relates to a preparation method and an application of a composite nanometer material for the combined treatment of tumor by optothermal and active oxygen, and belongs to the technical field of medicines used for treatment. Nanometer iron is prepared through an ethylene glycol method, a catechol group on a dopamine molecular is oxidized under the condition of alkaline solution and oxygen, dyhydroxy benzpyrole or a semiquinone free radical is formed to generate rearrangement or coupling crosslinking auto polymerization reaction, a polydopamine layer coats the outer surface of the nanometer iron, the active group of the polydopamine is used for realizing covalent linkage with glucose oxidase to prepare a nanometer iron / polydopamine / glucose oxidase (nanometer iron / PDA(propane diamine) / GOx(gaseous oxygen)) composite nanometer material, and the composite material of the inorganic nanometer material modified by biomacromolecules and biomimetic materials is constructed. The prepared material has the advantages of obvious treatment effect, high biocompatibility, no toxicity and no pollution. The preparation technology has the advantages of simpleness and low cost and is suitable for scale production and clinical medical care.

Description

technical field [0001] The invention relates to a preparation method and application of a composite nanomaterial for the combined treatment of tumors with photothermal and active oxygen, and belongs to the technical field of therapeutic drugs. Background technique [0002] In cancer treatment, in order to improve the anti-tumor effect and reduce the toxic and side effects, it is an important strategy for cancer treatment to develop compound therapeutic agents targeting the weakness of tumor tissue. Tumor cells are very dependent on glucose due to their rapid growth and proliferation. Moreover, due to its unique tissue structure and metabolic characteristics, tumors are much less resistant to heat (>42°C) and exogenous reactive oxygen species than normal cells; Macromolecules and nanomaterials with a certain particle size (100-200 nm) have significant retention and accumulation effects (enhanced permeability and retention effect, EPR). [0003] Photothermal therapy is a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61P35/00
CPCA61K41/0052A61K41/0057A61K2300/00
Inventor 徐莉张甜甜肖敏李银萍洪伟英
Owner SHANDONG UNIV
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