Preparation method and application of earthworm antibacterial peptide

A technology of antibacterial peptides and earthworms, applied in the fields of peptide preparation methods, antibacterial drugs, chemical instruments and methods, etc.

Inactive Publication Date: 2021-04-20
广东莞垄农业生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, earthworms have not been applied to production as an antibacterial inhibitor at present.

Method used

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Embodiment Construction

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them.

[0017] The preparation method of a kind of earthworm antimicrobial peptide proposed by the present invention comprises the following steps:

[0018] S1, S1, and earthworms were injected with a little 0.05mol / L phosphate buffer solution into the beaker during 3-5h ultrasonic induction;

[0019] S2, after the induction time is completed, the earthworms are shocked with a voltage of 5V, and the yellow liquid secreted by the earthworms after the electric shock is collected with a pipette;

[0020] S3, extract the yellow liquid secreted by S2 earthworms into a 1.5ml centrifuge tube, centrifuge at 3000r / min for 30min at 4°C,

[0021] S4. Collect the supernatant after centrifugation in S3, and put it into a -80°C refrigerator for freezing;

[0...

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PUM

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Abstract

The invention discloses a preparation method of earthworm antibacterial peptide. The preparation method comprises the following steps: S1, injecting a small amount of 0.05 mol / L phosphate buffer solution into a beaker when earthworms are subjected to ultrasonic induction for 3-5 hours; S2, after the induction is finished, electrically shocking the earthworms with 5V voltage, and collecting yellow liquid secreted by the earthworms after electric shock with a pipettor; S3, extracting yellow liquid secreted by earthworms in the S2, putting the yellow liquid into a 1.5 ml centrifugal tube, and carrying out centrifuging for 30 minutes at 2-6 DEG C and 3000 r / min; S4, collecting supernatant obtained after centrifugation in the S3, and putting the supernatant into a refrigerator at subzero100-subzero 75 DEG C for cryopreservation; and S5, transferring crude extract cryopreserved in the step S4 into a vacuum freeze dryer for concentration, taking out the crude extract after drying, and quickly putting the crude extract into a refrigerator at subzero 80 DEG C for cryopreservation. The earthworm antibacterial peptide has good bacterial growth inhibition activity, can kill or inhibit bacteria, and has broad-spectrum antibacterial characteristics which are incomparable to those of traditional antibiotics. The antibacterial peptide interacts with a bacterial cell membrane with a charge neutralization method to penetrate and kill bacteria, and therefore, the possibility of drug resistance of the bacteria is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of antimicrobial peptide preparation, in particular to a preparation method and application of an antimicrobial peptide of dilong. Background technique [0002] There are many types of soil / sewage pollutants, analyzed according to their nature: mainly divided into chemical pollutants / physical pollutants / biological pollutants / radioactive pollutants, including inorganic pollutants and organic pollutants, involving mercury, cadmium, lead, arsenic Heavy metals such as heavy metals and excessive nitrogen / phosphorus plant nutrients and oxides, sulfides and fluorides; involving various chemical pesticides, agricultural films, packaging, herbicides, detergents, petroleum and its cracking products and phenols, etc., including Organic synthesis products and domestic industrial and mining pollutants, etc. The present invention uses earthworms (also known as earthworms) to naturally and dynamically catalyze and absorb ...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K2/00A61P31/04
Inventor 孔作万叶世豪黄挺薛红
Owner 广东莞垄农业生态科技有限公司
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