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Endometrial polyp methylation marker combination, detection kit and application

A technology of endometrial polyps and gene markers, applied in the field of medical diagnosis, can solve the problems of high trauma, time cost and economic cost

Active Publication Date: 2021-04-30
SHENZHEN CITY BAOAN DISTRICT MATERNAL & CHILD HEALTH HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a method requires the patient to go to a professional medical institution for examination by a professional doctor, which has high time and economic costs, and hysteroscopy will also cause certain trauma

Method used

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  • Endometrial polyp methylation marker combination, detection kit and application
  • Endometrial polyp methylation marker combination, detection kit and application
  • Endometrial polyp methylation marker combination, detection kit and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Gene marker panel, including IGF1R, CTBP1, TCF7L1, E2F3, CACNA2D4, KCNJ12, TPO, UGT1A8, UGT1A10, CABP5, CST9, ITGA2, DLGAP2, ESPNP, NBPF25P, RASA3, ZIM2, PXDN, HDAC4 and VAV2, the gene markers The methylation levels of the samples were used to diagnose endometrial polyps.

Embodiment 2

[0039] Primer sets for detection of gene markers including IGF1R, CTBP1, TCF7L1, E2F3, CACNA2D4, KCNJ12, TPO, UGT1A8, UGT1A10, CABP5, CST9, ITGA2, DLGAP2, ESPNP, NBPF25P, RASA3, ZIM2, PXDN, HDAC4 and VAV2. The primer sequences in the primer set are shown in SEQ ID NO: 1-40.

[0040]

[0041]

[0042]

[0043]

[0044]

[0045] experimental method

[0046] Collect 100 plasma samples from patients with clinical endometrial polyps and healthy subjects (from Shenzhen Baoan District Maternal and Child Health Hospital) for detection. The detection method is as follows:

[0047] 1. Methylation treatment

[0048] Extract the cfDNA of the specimen and configure the TET2 treatment system: cfDNA 2ng; TET2 Reaction Buffer 2.5μl; Oxidation Supplement 0.25μl; Oxidation Enhancer 0.25μl; TET21μl; The conditions are: incubate at 37°C for 1 hour. Add 0.25ul Stop Reagent to the TET2-treated product; the reaction conditions are: incubate at 37°C for 30min. After the reaction w...

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Abstract

The invention provides an endometrial polyp methylation marker combination for the first time. The endometrial polyp methylation marker combination comprises the following gene markers: IGF1R, CTBP1, TCF7L1, E2F3, CACNA2D4, KCNJ12, TPO, UGT1A8, UGT1A10, CABP5, CST9, ITGA2, DLGAP2, ESPNP, NBPF25P, RASA3, ZIM2, PXDN, HDAC4 and VAV2; the endometrial polyp can be effectively diagnosed by detecting the methylation level of the gene markers in a sample to be detected from a subject, the specificity reaches 87.27%, and the sensitivity reaches 91.11%.

Description

technical field [0001] The invention relates to the field of medical diagnosis, and more specifically, to a combination of endometrial polyp methylation markers, a detection kit and applications. Background technique [0002] Endometrial polyps (endometrial polyps) is a common disease in gynecology, which is caused by local excessive hyperplasia of endometrium, manifested as single or multiple smooth masses protruding from the uterine cavity, with different pedicles; Vaginal bleeding, infertility. Women from childbearing age to postmenopausal women are all high-risk groups of endometrial polyps. The probability of women suffering from endometrial polyps in their lifetime is about 70%. Among these patients, about 15% occur in the reproductive age stage. Endometrial polyps in the reproductive age stage may lead to infertility. [0003] Commonly used examination methods for endometrial polyps are gynecological examination, ultrasonography, hysteroscopy, and pathological exami...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/154Y02A50/30
Inventor 刘斌夏渝东陆美茵郑子鹏李晓红沈俊芳
Owner SHENZHEN CITY BAOAN DISTRICT MATERNAL & CHILD HEALTH HOSPITAL
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