Detection system for urinary tract infection pathogen drug resistance genes and kit and application thereof

Abstract

The invention relates to a detection system for urinary tract infection pathogen drug resistance genes and a kit and application thereof. The detection system has 10 pairs of primers, including 9 pairs of drug resistance gene primers and 1 pair of system quality control internal reference primers. The urinary tract infection pathogen drug resistance genes aim at an ESBLs genotype, a carbapenemase gene, an MRSA genotype and a VRE genotype. According to the detection system for the urinary tract infection pathogen drug resistance genes and the kit and application thereof, conventional detection is not needed, synchronous detection and analysis of various pathogen drug-resistant genes can be directly carried out on a urine sample in the same reaction system, the defects of low flux, long time consumption and the like of a conventional detection method are overcome, comprehensive, accurate and low-cost etiological diagnosis is provided for clinics at first time, and important reference is provided for individualized medication and accurate medical treatment.

Description

Technical field[0001]The present invention relates to a multi-gene detection product and the detection system used in the product belongs to the field of biotechnology.Background technique[0002]Urinary tract infection is also known as urinary tract infections, UTI, is one of the most common infective diseases in the clinic, secondary infection infection infection. There are a wide variety of pathogens in UTI, and pathogens that are often separated from urine samples are sequentially Escherichia coli, pneumoniae, pneumoniae, pneumococcus, copper pseudomonas aerugin, white candida, singular deformation bacilli and no Blue bacteria and the like. The most effective treatment of urinary tract infection is to use antibiotic treatment, but with the general and excessive use of antibiotics, the drug resistance of common antibiotics has gradually increased, and the common bacteria of urinary tract infection is in the drug resistance of antibiotics. It is quite common in the population. Altho...

AI Technical Summary

Problems solved by technology

However, the current conventional detection methods have the disadvantages of low detection rate, long time-consuming, especially the inability to accurately identify multiple pathogens at the same time, and cannot provide timely, comprehensive and accurate pathogen diagnosis basis for clinical practice, leading to the widespread use of broad-spectrum Empirical treatment with antibacterial drugs leads to low curative effect, failure to control the progress of urinary tract infection in time, high drug resistance rate of induced strains, and increased medical burden on patients, etc.

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