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Application of Small Molecular Compounds in Promoting Plant Growth and Development

A small molecular compound, a technology to promote plant growth, applied in the direction of plant growth regulators, plant growth regulators, applications, etc., can solve problems such as the inability to achieve tissue or organ specificity

Active Publication Date: 2022-02-15
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on this, the present invention has discovered a small molecular compound through research, which has tissue or organ specificity to the growth and development of plants, and uses the distribution mechanism of plant endogenous auxin in growth and development to balance the effects of various tissues and organs on the growth and development of plants. The need for auxin, addressing the inability of current auxin-based products to achieve tissue or organ specificity

Method used

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  • Application of Small Molecular Compounds in Promoting Plant Growth and Development
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  • Application of Small Molecular Compounds in Promoting Plant Growth and Development

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specific Embodiment

[0062] The following description will be described in detail below. The following implementations are not particularly illustrated, other components except unavoidable impurities are not included. In an embodiment, reagents and instruments are used as non-specifically descriptions, are conventionally selected in the art. The experimental method of the specific condition is not indicated in the examples, and the conditions described in conventional conditions, such as literature, books described in the books or manufacturers recommended.

[0063] It should be noted that in the following embodiments, the structure is The small molecule compound is simimer as "C9", and C9 is synthesized by Shanghai NFC, and the medium is added to the medium after dissolving when using DMSO. In this article, "μm" refers to "μmol / L"; PPBO is 4-Phenoxyphenylboronic ACID; L-KYN is L-KYNURENINE, the growth of the synthesis pathway IPA pathway inhibitor; IAA refers to indole-3-acetic acid; The composit...

Embodiment 1

[0065] Effects of Different Concentration C9 on the Subopolymerization of Arabidopsis

[0066] (1) Place the Arabidopsis seeds (Colombian Type 0, COL-0) were seeded on the medium of C9 group, IAA group and blank control group, and cultured for 6 days, and 30 of each group was planted. Among them, the medium of the C9 group is a C9 containing 0.1 μm, 0.3 μm, 0.8 μm, 1.5 μm, 3 μm, and 5 μm, respectively. The medium of the IAA group is an IAA containing 0.05 μm, 0.1 μm, 0.3 μm and 1 μm, respectively. The medium of the blank control group did not process Medium. Of course, the C9 group, the IAA group, and the other culturing conditions of the blank control group were the same.

[0067] (2) After the end of culture, the growth status of the medal mustard in each group was observed, record the length of the dominant root length of the intertworm, the length of the lower embryo shaft, and the quantitative treatment of different IAA concentration treatment under different C9 concentrat...

Embodiment 2

[0074] The effect of C9 on the number of side roots in Arabidopsis

[0075] (1) will The medium was randomly divided into three groups of 5 days and aging agonism (Colombia 0, Col-0): C9 group, IAA group and blank control group.

[0076] (2) Transfer of the c9 groups to the Templea Temple to contain 0.8 μm and 1.5 μm C9 The treatment plate of the medium was cultured for 3 days. Similarly, transfer of the IAA group in the IAA group to IAA containing 0.3 μm The treatment plate of the medium was cultured for 3 days. Arabidopausica in the blank control group is transferred to the process board The medium was cultured for 3 days to cultivate 3 days. Of course, the C9 group, the IAA group, and the other culturing conditions of the blank control group were the same. After the end, the plants and side roots of the intertwood of each group were recorded. Figure 5 and Image 6 Indicated. Figure 5 For C9 groups, the IAA group and the blank control group (the MOCK group in the figure) were ...

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Abstract

The invention relates to the application of a small molecular compound in promoting plant growth and development. The structural formula of the small molecular compound is that the above small molecular compound can inhibit the amino acidization of plant endogenous auxin by inhibiting the activity of plant GH3 protein, so that the accumulation of auxin in plants presents the original tissue or organ specificity, which is beneficial to plant growth development.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to an inhibitor of GH3 proteins in regulatory plant growth and development. Background technique [0002] The growth of the growth is a class of endogenous hormones containing an unsaturated aromatic ring and a acetic acid side chain, and the chemical essence is indole acetic acid. Indole-3-acetic acid (IAA) is the most important form of growth in plants. It regulates multiple aspects of plant growth development, such as regulating plant cell splitting and elongation, plant direction and top advantage, side roots and indide roots Forming, blade falling and fruit development, etc. The regulation of growthins mainly depends on the concentration of growthins, excessive or too low growth, will have an adverse effect on the plant. Thus, plant growthin concentration regulatory research is of great significance for agricultural production applications. [0003] After nearly 20 years of rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/58A01P21/00A01G24/30
CPCA01N43/58A01G24/30
Inventor 郭红卫谢银鹏姜凯朱颖
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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